Awesome presentation! Do you think silver nanoparticles kill many types of bacteria or just Salmonella? Do you think it kills bacteria in the same way as ampicillin, or do you think silver nanoparticles would kill ampicillin-resistant bacteria? How would you test these things?
I think the silver annoparticles would kill many other type of bacteria. Most of the research we came across tested silver nanoparticles against E.coli and were successful in killing it. I think silver nanoparticles would kill ampicillin resistant bacteria because the mechanism of how silver ions kill bacteria is different from that of ampicillin. I would test different types of bacteria against both silver nanoparticles and ampicillin to see what how they compare, if either or both could inhibit or kill a given bacteria.
Good job! The talking was clear and it was easy to listen to. Maybe you could go into more detail on the methods and explain the implications a little more.
My favorite experiment was the bacteriostatic v. bactericidal experiment because it determined whether or not silver nanoparticles killed or inhibited the growth of S. Typhimurium which was cool to learn that different compound could only inhibit growth of a bacteria but that information is still useful in antibiotic research and treatment for infections.
For both the max-dose and dose response experiments, a maximum concentration of 10 mg/mL was tested, which is the maximum amount allowed within the human body. The dose-response was a serial dilution experiement at 50%, until the concentration reached 0.625 mg/mL. The Bacteriostatic v. Bactericidal experiment incubated the S. Typhimurium in a well of the negative contorl, postive contol, and silver for 24 hours then transferred what was in those wells to new media and incubated again to check for any new growth.
All three tests, the max-dose, the dose-response, and bacteriostatic v. bactericidal experiements, all yielded statistical hits; silver had an effect on the S. Typhimurium and it infact killed it. However, the dose response did not show at what dose silver would be ineffective. All of the doses were equally effective at killing the bacteria. This could mean that while the compound can kill bacteria it may also kill or damage cells that are necassary if it was in a living organism, which is a concern regarding silver ion toxicity.
Really cool interesting presentation!!! Sorry if I missed it but did you say that silver nanoparticles could be dangerous to humans?? If so would it be extremely important to understand the dosage to a very exact number so it isn’t harmful.
Yes, silver nanoparticles and nanoparticles in general could be dangerous in vivo. It would be important to find out how much silver is toxic in the body, and if inside the body, would it act more like a poison, rather than being beneficial, like it is outside of the body.
Great job I thought this was a great presentation. I know you spoke of this a little bit in your video but how exactly is silver a disinfectant? What happens at the molecular level between the silver nanoparticles and the bacteria?
Silver is able to degrade the cell membrane and interfere with DNA binding, splitting it into two separate strands instead of one.
Silver is used as a disinfectant because of its ability to act as a antimicrobial agent; it degrades bacteria and can sometimes be included in food packaging or used in place of other disinfectants if they corrode surfaces or cause toxic by-products.
Great poster and video! I know you spoke a little of this in your video but how exactly do the silver nanoparticles kill the bacteria? At the molecular level?
Great presentation! You said this experiment can’t determine the toxicity of the silver nanoparticles. How would you test if It is poison if you were to do it in future experiments ?
I would continue testing lower doses or other forms (liquid or powder etc.) of silver ions in future experiments. I would also recommend further experiements on how exactly silver ions, at the molecular level, have a toxic effect on a cell if it was determined to be a poison.
I think first testing how exactly silver nanoparticles are causing toxic effects in any cell, the molecular mechanisms of that, and then testing the silver on actual somatic cells would be a way to test how they are toxic or if they are toxic to somatic cells specifically, or if it is just to bacterial cells.
curesymposium.org 
Awesome presentation! Do you think silver nanoparticles kill many types of bacteria or just Salmonella? Do you think it kills bacteria in the same way as ampicillin, or do you think silver nanoparticles would kill ampicillin-resistant bacteria? How would you test these things?
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I think the silver annoparticles would kill many other type of bacteria. Most of the research we came across tested silver nanoparticles against E.coli and were successful in killing it. I think silver nanoparticles would kill ampicillin resistant bacteria because the mechanism of how silver ions kill bacteria is different from that of ampicillin. I would test different types of bacteria against both silver nanoparticles and ampicillin to see what how they compare, if either or both could inhibit or kill a given bacteria.
LikeLike
Good job! The talking was clear and it was easy to listen to. Maybe you could go into more detail on the methods and explain the implications a little more.
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What was your favorite experiment that you did?
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My favorite experiment was the bacteriostatic v. bactericidal experiment because it determined whether or not silver nanoparticles killed or inhibited the growth of S. Typhimurium which was cool to learn that different compound could only inhibit growth of a bacteria but that information is still useful in antibiotic research and treatment for infections.
For both the max-dose and dose response experiments, a maximum concentration of 10 mg/mL was tested, which is the maximum amount allowed within the human body. The dose-response was a serial dilution experiement at 50%, until the concentration reached 0.625 mg/mL. The Bacteriostatic v. Bactericidal experiment incubated the S. Typhimurium in a well of the negative contorl, postive contol, and silver for 24 hours then transferred what was in those wells to new media and incubated again to check for any new growth.
All three tests, the max-dose, the dose-response, and bacteriostatic v. bactericidal experiements, all yielded statistical hits; silver had an effect on the S. Typhimurium and it infact killed it. However, the dose response did not show at what dose silver would be ineffective. All of the doses were equally effective at killing the bacteria. This could mean that while the compound can kill bacteria it may also kill or damage cells that are necassary if it was in a living organism, which is a concern regarding silver ion toxicity.
LikeLike
Really cool interesting presentation!!! Sorry if I missed it but did you say that silver nanoparticles could be dangerous to humans?? If so would it be extremely important to understand the dosage to a very exact number so it isn’t harmful.
LikeLike
Yes, silver nanoparticles and nanoparticles in general could be dangerous in vivo. It would be important to find out how much silver is toxic in the body, and if inside the body, would it act more like a poison, rather than being beneficial, like it is outside of the body.
LikeLike
Great job I thought this was a great presentation. I know you spoke of this a little bit in your video but how exactly is silver a disinfectant? What happens at the molecular level between the silver nanoparticles and the bacteria?
LikeLike
Silver is able to degrade the cell membrane and interfere with DNA binding, splitting it into two separate strands instead of one.
Silver is used as a disinfectant because of its ability to act as a antimicrobial agent; it degrades bacteria and can sometimes be included in food packaging or used in place of other disinfectants if they corrode surfaces or cause toxic by-products.
LikeLike
Great poster and video! I know you spoke a little of this in your video but how exactly do the silver nanoparticles kill the bacteria? At the molecular level?
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Hello, great poster and video! I know you spoke of this a little but how exactly do the silver nanoparticles kill the bacteria at the molecular level?
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Great presentation! You said this experiment can’t determine the toxicity of the silver nanoparticles. How would you test if It is poison if you were to do it in future experiments ?
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I would continue testing lower doses or other forms (liquid or powder etc.) of silver ions in future experiments. I would also recommend further experiements on how exactly silver ions, at the molecular level, have a toxic effect on a cell if it was determined to be a poison.
LikeLike
Hi, great presentation! How could you determine if silver nanoparticles are toxic to somatic cells
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I think first testing how exactly silver nanoparticles are causing toxic effects in any cell, the molecular mechanisms of that, and then testing the silver on actual somatic cells would be a way to test how they are toxic or if they are toxic to somatic cells specifically, or if it is just to bacterial cells.
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