Took me awhile to come up with a question because you answered everything very clearly each time something popped into my head! I was wondering if any other marine life apart from cuttlefish ink shows a decrease in bacterial gross?
Took me awhile to come up with a question since you answered everything very clearly! I was just wondering if there is evidence on other marine life besides cuttlefish ink that shows a decrease in bacterial growth?
Thank you! I know that cephalopods in general have shown a decrease in bacterial growth. Specifically, squid ink has a presence as an antimicrobial compound in some countries.
I could’ve missed it but is there a reason the threshold of antibiotic resistance is around 2 standard deviations, and what structural characteristics add to antibiotics?
Two standard deviations will give a 2.5% chance of identifying unusual compounds – whether causing or limiting growth. This is an industry standard as well. I’m not sure what specific structural characteristics add to antibiotics, only that functional groups affect the function of the antibiotic.
Retesting with less bacteria would allow the cuttlefish ink to show its properties because it would not represent as “strong” of an infection, and thus the compound might be more effective.
Good presentation. Do you think that the vortexing that you did in order to create your stock solution may have played a role on why your data may not have shown up as a hit? Also, why did you vortex your dilution series if you had already vortexed the stock solution?
Thank you! I don’t think that vortexing the stock solution played a role in our data not showing up as a hit, because by vortexing, we ensured the the cuttlefish ink was evenly dispersed within the stock solution, and that none of the ink was left settled at the bottom. We vortexed the dilution series as well to ensure that each of the vials receiving solution from previous vials got a dose of solution with evenly dispersed ink so that the measurements would show proportionate amounts of compound and solvent (ethanol). Vortexing was important because after a short period of time the ink would settle to the bottom, and thus without vortexing, the pipette would only draw up ethanol solvent.
Very well spoken! great job! when you say “Test individual compounds identified in the cuttlefish ink extract” which curoirs as to what compounds are you talking about?
Thank you! When isolating different compounds in the ink, it is critical to separate the antimicrobial compounds from those that provide nutrients so that the bacteria doesn’t have access to nutrients.
curesymposium.org 
Took me awhile to come up with a question because you answered everything very clearly each time something popped into my head! I was wondering if any other marine life apart from cuttlefish ink shows a decrease in bacterial gross?
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Took me awhile to come up with a question since you answered everything very clearly! I was just wondering if there is evidence on other marine life besides cuttlefish ink that shows a decrease in bacterial growth?
LikeLike
Thank you! I know that cephalopods in general have shown a decrease in bacterial growth. Specifically, squid ink has a presence as an antimicrobial compound in some countries.
LikeLike
I could’ve missed it but is there a reason the threshold of antibiotic resistance is around 2 standard deviations, and what structural characteristics add to antibiotics?
LikeLike
Two standard deviations will give a 2.5% chance of identifying unusual compounds – whether causing or limiting growth. This is an industry standard as well. I’m not sure what specific structural characteristics add to antibiotics, only that functional groups affect the function of the antibiotic.
LikeLike
What would retesting with less bacteria do for your results?
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Retesting with less bacteria would allow the cuttlefish ink to show its properties because it would not represent as “strong” of an infection, and thus the compound might be more effective.
LikeLike
Good presentation. Do you think that the vortexing that you did in order to create your stock solution may have played a role on why your data may not have shown up as a hit? Also, why did you vortex your dilution series if you had already vortexed the stock solution?
LikeLike
Thank you! I don’t think that vortexing the stock solution played a role in our data not showing up as a hit, because by vortexing, we ensured the the cuttlefish ink was evenly dispersed within the stock solution, and that none of the ink was left settled at the bottom. We vortexed the dilution series as well to ensure that each of the vials receiving solution from previous vials got a dose of solution with evenly dispersed ink so that the measurements would show proportionate amounts of compound and solvent (ethanol). Vortexing was important because after a short period of time the ink would settle to the bottom, and thus without vortexing, the pipette would only draw up ethanol solvent.
LikeLike
Very well spoken! great job! when you say “Test individual compounds identified in the cuttlefish ink extract” which curoirs as to what compounds are you talking about?
LikeLike
Thank you! When isolating different compounds in the ink, it is critical to separate the antimicrobial compounds from those that provide nutrients so that the bacteria doesn’t have access to nutrients.
LikeLike