great question! having a clear and solid border tell us that all of the bacteria in that area has been killed and nothing was left alive which tells us the virus has a lytic life cycle instead of lysogenic
Were you able to do a PCR or gel electrophoresis to identify the family of the phage? If not, do you have any predictions for which family it might belong to?
that’s a great thought but probably not. weather or not a bacteria can be a host for a virus is 100% dependent on the surface cell receptors which are not linked to the life cycle mechanisms as far as i know.
Yes! if it is lytic it will kill everything it infects. This means all bacteria that host it will die. This is actually preferred when we are looking for phage therapy candidates because it means the phage will lot leave behind some living bacteria in its wake.
Hello! Great presentation! I was curious what the importance was for having a clear inside and solid borders as described in figure 2. Thank you!
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great question! having a clear and solid border tell us that all of the bacteria in that area has been killed and nothing was left alive which tells us the virus has a lytic life cycle instead of lysogenic
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Hello! Great presentation! I was wondering what the importance was for having a clear inside and solid borders as described in figure 2. Thank you!
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Hi!
What is Siphoviridae and what implications does this have?
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Siphoviridae is a family of double-stranded DNA viruses in the order Caudovirales. it is determined by the body type of the phage
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Hi, I was wondering what Siphoviridae means and what implications this has to research?
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Were you able to do a PCR or gel electrophoresis to identify the family of the phage? If not, do you have any predictions for which family it might belong to?
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we got as far as a gel electrophoresis but the quality control was either too low quantity or quality in both attempts.
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Do you think your phage’s lytic nature/identity could be used to find other genetically similar bacteria to have host it?
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that’s a great thought but probably not. weather or not a bacteria can be a host for a virus is 100% dependent on the surface cell receptors which are not linked to the life cycle mechanisms as far as i know.
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Hello! Could you explain a little more how having a lytic life cycle influences its effect on bacteria?
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Yes! if it is lytic it will kill everything it infects. This means all bacteria that host it will die. This is actually preferred when we are looking for phage therapy candidates because it means the phage will lot leave behind some living bacteria in its wake.
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