They sometimes can have a cloudy halo or “turbid plaque” because if they go through the lysogenic cycle at a high enough frequency then this appearance is created.
Were you able to estimate the size of your electrophoresis bands? It looks like the DNA ladder is mostly below your products so it’s kind of hard to see on my screen.
The PCR tests conducted were the quality control test, which was highly concentrated enough with little degradation and the restriction digest. We estimate that for our quality control that it was around 16 ng/5microliters, and for the restriction digest, we never looked into estimating where the cuts were. We were more focused on finding the cluster.
Host cell replication makes lysogenic phages usable for phage therapy because they do not kill the host cell. In phage therapy, the host cell is the harmful bacteria, so if the phage is unable to kill it, it can’t be utilized.
The impact of UV radiation is just another experiment that is being done nowadays with phages. It is important because if phage DNA replication is not negatively impacted by UV lights then it can be used in phage therapy in crops. Due to global warming bacterial illnesses in plants have been on the rise, so this could be a possible solution.
curesymposium.org 
Why do lysogenic phages present with a cloudy halo around them?
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They sometimes can have a cloudy halo or “turbid plaque” because if they go through the lysogenic cycle at a high enough frequency then this appearance is created.
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What would cause a lysogenic bacteriophage to enter the lytic cycle?
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If the phage is exposed to DNA damaging agents, including UV radiation; the phage DNA could exit the chromosome and move into the lytic cycle.
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Were you able to estimate the size of your electrophoresis bands? It looks like the DNA ladder is mostly below your products so it’s kind of hard to see on my screen.
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The PCR tests conducted were the quality control test, which was highly concentrated enough with little degradation and the restriction digest. We estimate that for our quality control that it was around 16 ng/5microliters, and for the restriction digest, we never looked into estimating where the cuts were. We were more focused on finding the cluster.
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Thank you for sharing your work. One question I have is why host cell replication during the lysogenic cycle prevent it for being used in therapy?
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Host cell replication makes lysogenic phages usable for phage therapy because they do not kill the host cell. In phage therapy, the host cell is the harmful bacteria, so if the phage is unable to kill it, it can’t be utilized.
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Why is it important to see if UV radiation affects phage function and replication?
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The impact of UV radiation is just another experiment that is being done nowadays with phages. It is important because if phage DNA replication is not negatively impacted by UV lights then it can be used in phage therapy in crops. Due to global warming bacterial illnesses in plants have been on the rise, so this could be a possible solution.
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