The lysogenic lifecycle can only occur in temperate phages. The lysogenic lifescycle is when the bacteriophage integrates it’s DNA into the host’s DNA in order to continue replication while the lytic lifecycle destroys the host’s DNA in order to replicate.
Strong presentation! You said in your future directions section you would investigate the HAE3 enzyme because there were no cuts, why do you think that is?
This is could be a result of our restriction enzymes not incubating for the full 24 hours. We were kind of rushed towards the end so our results were somewhat inconclusive. However, this is could also be because our phage is apart of a cluster that isn’t cut by that restriction enzyme.
Hello! In the future directions you discussed sequencing your phages dna to identify what causes different sized plaques. I was wondering if there was a major difference in the data based on the size of the plaque and if this could serve multiple functions?
As far as we know, there was no major difference in our data dependent on our plaque size. Our high titer lysate contained both plaque sizes even though there was a clear indication only one phage was isolated. This could be due to the fact we had a temperate phage. Our temperate phage could serve to create vaccines because of its ability to increase bacterial virulence or used in phage therapy if the phage was modified to only go through the lytic lifecycle.
You discuss the phages potential for contributing to antibiotic resistant therapies… I was wondering how do you go about testing this potential further down the line? Are these bacteriophages introduced to MDR bacteria that we see in healthcare settings, for example, MRSA, and seeing if they lyse?
These bacteriophages are being introduced to different patients in order to see if they are able to lyse and defeat antibiotic resistance in patients with conditions that are resistant to antibiotics. As far as testing down the line, I think we would have to further our DNA sequencing to determine completely the cluster of our phage in order to really know if we have the potential to utilize our phage in vaccines or in phage therapy.
curesymposium.org 
What are the main differences between the lysogenic and lytic life cycles?
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The lysogenic lifecycle can only occur in temperate phages. The lysogenic lifescycle is when the bacteriophage integrates it’s DNA into the host’s DNA in order to continue replication while the lytic lifecycle destroys the host’s DNA in order to replicate.
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Strong presentation! You said in your future directions section you would investigate the HAE3 enzyme because there were no cuts, why do you think that is?
LikeLike
This is could be a result of our restriction enzymes not incubating for the full 24 hours. We were kind of rushed towards the end so our results were somewhat inconclusive. However, this is could also be because our phage is apart of a cluster that isn’t cut by that restriction enzyme.
LikeLike
Hello! In the future directions you discussed sequencing your phages dna to identify what causes different sized plaques. I was wondering if there was a major difference in the data based on the size of the plaque and if this could serve multiple functions?
LikeLike
As far as we know, there was no major difference in our data dependent on our plaque size. Our high titer lysate contained both plaque sizes even though there was a clear indication only one phage was isolated. This could be due to the fact we had a temperate phage. Our temperate phage could serve to create vaccines because of its ability to increase bacterial virulence or used in phage therapy if the phage was modified to only go through the lytic lifecycle.
LikeLike
You discuss the phages potential for contributing to antibiotic resistant therapies… I was wondering how do you go about testing this potential further down the line? Are these bacteriophages introduced to MDR bacteria that we see in healthcare settings, for example, MRSA, and seeing if they lyse?
LikeLike
These bacteriophages are being introduced to different patients in order to see if they are able to lyse and defeat antibiotic resistance in patients with conditions that are resistant to antibiotics. As far as testing down the line, I think we would have to further our DNA sequencing to determine completely the cluster of our phage in order to really know if we have the potential to utilize our phage in vaccines or in phage therapy.
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