We had gone back after still getting two different sized plaques to try purifying again, but still ended up with the same results. We could potentially go back and attempt to purify the phage again, but we would most likely go with the phages we have now since we had already tried purifying them again.
One explanation that we though of for the consistent discrepancy is that the phages took longer to integrate its DNA into the host cell and by the time we were looking at the plates for results, they were still integrating their DNA into the bacteria. Completing DNA sequencing on our phage may help consolidate whether or not we actually have only one phage that presents itself with different sized plaques.
Sequencing the DNA would be a better indicator of whether or not we have one type of phage. We were able to get back EM photos of our phage which indicates that there is possibly only one phage, but we are still unclear of the reasoning behind the different plaque sizes.
curesymposium.org 
If you were to sequence the DNA would you need to purify the phages again so that they were all the same size or go with the plaques you have now?
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We had gone back after still getting two different sized plaques to try purifying again, but still ended up with the same results. We could potentially go back and attempt to purify the phage again, but we would most likely go with the phages we have now since we had already tried purifying them again.
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What are some possibilities for the reason that your phages were consistently two sizes, and how would a DNA sequence make the answer clearer?
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One explanation that we though of for the consistent discrepancy is that the phages took longer to integrate its DNA into the host cell and by the time we were looking at the plates for results, they were still integrating their DNA into the bacteria. Completing DNA sequencing on our phage may help consolidate whether or not we actually have only one phage that presents itself with different sized plaques.
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When you mentioned the different sizes in the phages inside of the DNA, If the DNA was more similar in size would the DNA sequence make it clearer?
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Sequencing the DNA would be a better indicator of whether or not we have one type of phage. We were able to get back EM photos of our phage which indicates that there is possibly only one phage, but we are still unclear of the reasoning behind the different plaque sizes.
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