We don’t know the exact lysing rate of our phage that could be something to do further research on but we do know that our plaques (formed from lysed cells) were showing up after about a 48 hour incubation period after we first isolated our phage.
We do a procedure called enrichment. Once obtaining a soil sample, we put it in a flask with LB media and M. smeg, the bacteria used in our experiment. We then incubated the flask so that the phage would (hopefully) start infecting the bacteria. After the incubation period, using a sample of the liquid in the flask and did spot test and plaques showed up on the plate, meaning myobacteriophages were present and infecting the M.smeg. If plaques don’t show up the enrichment process would be done all over again.
curesymposium.org 
Nice presentation! How long would it take for the host cell to die after the phage injects its DNA into it?
LikeLike
We don’t know the exact lysing rate of our phage that could be something to do further research on but we do know that our plaques (formed from lysed cells) were showing up after about a 48 hour incubation period after we first isolated our phage.
LikeLike
Can you explain the soil prep/ how the phage was taken from the soil?
LikeLike
We do a procedure called enrichment. Once obtaining a soil sample, we put it in a flask with LB media and M. smeg, the bacteria used in our experiment. We then incubated the flask so that the phage would (hopefully) start infecting the bacteria. After the incubation period, using a sample of the liquid in the flask and did spot test and plaques showed up on the plate, meaning myobacteriophages were present and infecting the M.smeg. If plaques don’t show up the enrichment process would be done all over again.
LikeLike