The titer of a phage is the concentration of active phage within 1mL of lysate. When a phage is temperate it is living the lysogenic lifecycle. Thank you!
We did not expect to have a siphovirdae phage type morphology because we were under the impression that these morphologies were more rare. Our phage could have been either lysogenic or lytic it just depended on what phage we isolated from the others. For our high titer lysate we did expect a high titer because of the number of plaques we had observed on our plates. Lysogenic phages, also known as temperate phages, do create larger plaques so yes it is common for our phages lifecycle. Since they are so large and we have a high titer, it makes sense as to why our plaques are so close together and cover a lot of the agar plate. Thank you!
Phage only target bacterial cells They inject their DNA into the host bacteria cell and use the cell’s replication machinery to assembly more phage within the cell. These particles then burst through the host cell, breaking its membrane and killing it.
A siphovirdae phage has a long tail sheath and no tail fibers. Other phage morphologies can exclude a tail sheath or can have all of the phage parts shown in the presentation.
curesymposium.org 
What is a titer of a phage and what does it mean when a phage is temperate? I don’t know if that is the right word for it.
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The titer of a phage is the concentration of active phage within 1mL of lysate. When a phage is temperate it is living the lysogenic lifecycle. Thank you!
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Were these results what you expected? Are the plaques typically that large and close in proximity to one another (figure 5)? Great job btw!!
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We did not expect to have a siphovirdae phage type morphology because we were under the impression that these morphologies were more rare. Our phage could have been either lysogenic or lytic it just depended on what phage we isolated from the others. For our high titer lysate we did expect a high titer because of the number of plaques we had observed on our plates. Lysogenic phages, also known as temperate phages, do create larger plaques so yes it is common for our phages lifecycle. Since they are so large and we have a high titer, it makes sense as to why our plaques are so close together and cover a lot of the agar plate. Thank you!
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Were these results what you expected? Are the plaques typically that close in proximity to one another (figure 5)? Great job btw!!
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You said that phage can kill antibiotic resistant bacteria, how exactly compared to antibiotics?
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Phage only target bacterial cells They inject their DNA into the host bacteria cell and use the cell’s replication machinery to assembly more phage within the cell. These particles then burst through the host cell, breaking its membrane and killing it.
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How do you know if your phage is a siphoviridae phage or a different morphology?
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A siphovirdae phage has a long tail sheath and no tail fibers. Other phage morphologies can exclude a tail sheath or can have all of the phage parts shown in the presentation.
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