It actually not quite crystal clear. There is more research currently being done on temperate phages to see if we can determine which process but is it still not concrete.
There is no set and stone reason it is more of a question up in the air as to why this occurred. If we were able to have more time to research then, maybe we could have concluded as to why.
Hi Quintero!
Do you think it’s possible that the phage was contaminated, or that maybe your bacteria culture happened to randomly evolve at the time of experimentation leading to the temperate to lytic back to the temperate mutation?
We actually thought there was contamination when we had that switch from temperate to lytic and re did a spot test to see. When the results came back it was seen there was no contamination just more question as to know if the phage had mutated and if that was even possible!
Hi Caterina! Great job; I thought your presentation was super interesting. Do you have any predictions/ideas on what a mutating phage could contribute to phage therapy? How would this affect the phage’s effectiveness in infecting and killing bacteria?
Hi Jenny, great question! I think that mutating phage could be extremely useful for future research as knowing how to mutate a phage would allow a broader spectrum of phage to be used in phage therapy. Temperate phage are not usually considered as they have the opportunity to either kill the bacteria or insert their DNA and mutate it (making the infection possibly worse). If we were able to figure out how to mutate temperate phage to lytic, then we would have more species to chose from for phage therapy.
curesymposium.org 
What would cause the phage to go through lysis instead of lysogeny?
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It actually not quite crystal clear. There is more research currently being done on temperate phages to see if we can determine which process but is it still not concrete.
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Is there a reason that you had both lytic and temperate phages and not just one or the other?
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There is no set and stone reason it is more of a question up in the air as to why this occurred. If we were able to have more time to research then, maybe we could have concluded as to why.
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Hi Quintero!
Do you think it’s possible that the phage was contaminated, or that maybe your bacteria culture happened to randomly evolve at the time of experimentation leading to the temperate to lytic back to the temperate mutation?
LikeLike
We actually thought there was contamination when we had that switch from temperate to lytic and re did a spot test to see. When the results came back it was seen there was no contamination just more question as to know if the phage had mutated and if that was even possible!
LikeLike
Hi Caterina! Great job; I thought your presentation was super interesting. Do you have any predictions/ideas on what a mutating phage could contribute to phage therapy? How would this affect the phage’s effectiveness in infecting and killing bacteria?
LikeLike
Hi Jenny, great question! I think that mutating phage could be extremely useful for future research as knowing how to mutate a phage would allow a broader spectrum of phage to be used in phage therapy. Temperate phage are not usually considered as they have the opportunity to either kill the bacteria or insert their DNA and mutate it (making the infection possibly worse). If we were able to figure out how to mutate temperate phage to lytic, then we would have more species to chose from for phage therapy.
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