I really liked the posters pictures and the overall poster itself. I think it is very organized and precise. I think you did a great job at presenting the information very clearly and it showed that you knew what you were researching and presenting. Great Job!
I believe we had this shift because our plate used was left in the refrigerator for a long period of time which has been shown to induce a change like this before.
How sensitive do you think Mircat was to temperature changes? And do you think that its temperature sensitivity could be used to treat temperature based diseases/bacteria?
Mircat was possibly sensitive to temperature changes, but many phages are. There are ways to force Mircat to become lytic again and it could possibly be used as a phage therapy if this was done. However, it would not be effected by temperature at that point so it could not be used to treat temperature based bacteria.
Hi! I think your presentation was really good! My original questions have been said in a way in the other comments so I think those answers will be really interesting! Since you mentioned contamination as a reason plates 1 and 2 do not look the same, do you have any ideas on how that occurred or ways it could have been avoided. Also, just thought-provoking but how would you compare your specific phage to other phages in the cluster?
Thank you! Phages replicate very quickly and contamination between groups in the lab was not uncommon, however, testing of our lysate shows it did not have contamination. Our phage most likely shifted from lytic to temperate. Our phage did not clearly have a cluster so it would be difficult to compare to other phages in its cluster without sequencing Mircat’s genome.
Hi, I think this is a great poster! Do you believe that contamination happened? If you had more time with this experiment, would you have started all over or tried to continue working with Mircat?
Thanks! I do not believe that contamination occurred. I believe a lytic to temperate shift occurred despite its unlikelihood because none of the results were indicative that contamination occurred. If I had more time I would have loved to have tested the plaques from the lytic plate for homology with the temperate plaques which would completely rule out contamination.
Yes, at least one paper I found said that phage exposed to very cold environments for long periods of time can cause temperate phage to become lytic but Mircat went the opposite direction so the reason for this shift is still unclear.
curesymposium.org 
I really liked the posters pictures and the overall poster itself. I think it is very organized and precise. I think you did a great job at presenting the information very clearly and it showed that you knew what you were researching and presenting. Great Job!
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For your research, why do you think you experienced this shift in Mirat?
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I believe we had this shift because our plate used was left in the refrigerator for a long period of time which has been shown to induce a change like this before.
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How sensitive do you think Mircat was to temperature changes? And do you think that its temperature sensitivity could be used to treat temperature based diseases/bacteria?
LikeLike
Mircat was possibly sensitive to temperature changes, but many phages are. There are ways to force Mircat to become lytic again and it could possibly be used as a phage therapy if this was done. However, it would not be effected by temperature at that point so it could not be used to treat temperature based bacteria.
LikeLike
Hi! I think your presentation was really good! My original questions have been said in a way in the other comments so I think those answers will be really interesting! Since you mentioned contamination as a reason plates 1 and 2 do not look the same, do you have any ideas on how that occurred or ways it could have been avoided. Also, just thought-provoking but how would you compare your specific phage to other phages in the cluster?
LikeLike
Thank you! Phages replicate very quickly and contamination between groups in the lab was not uncommon, however, testing of our lysate shows it did not have contamination. Our phage most likely shifted from lytic to temperate. Our phage did not clearly have a cluster so it would be difficult to compare to other phages in its cluster without sequencing Mircat’s genome.
LikeLike
Hi, I think this is a great poster! Do you believe that contamination happened? If you had more time with this experiment, would you have started all over or tried to continue working with Mircat?
LikeLike
Thanks! I do not believe that contamination occurred. I believe a lytic to temperate shift occurred despite its unlikelihood because none of the results were indicative that contamination occurred. If I had more time I would have loved to have tested the plaques from the lytic plate for homology with the temperate plaques which would completely rule out contamination.
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Have you looked into any research regarding why a lytic phage would make this switch to temperate versus the other way around?
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Yes, at least one paper I found said that phage exposed to very cold environments for long periods of time can cause temperate phage to become lytic but Mircat went the opposite direction so the reason for this shift is still unclear.
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