What is the difference between the lysogenic cycle and the lytic cycle and how would these different processes effect your research differently? (i.e. if you had used a non-temperate phage instead)
The lysogenic cycle and lytic cycle are 2 parts of the same life cycle for bacteriophages. the lysogenic cycle reproduces its viral DNA with the prokaryotic bacteria, meaning the bacteria continues to live on as the phage DNA is being replicated. the lytic cycle is when a phage inserts its DNA and erodes the cell metabolism and its means of replication, it then synthesizes itself inside of the bacteria and when the new phages have matured, they burst through the cell killing it, which is called lysing the cell. the differences can be seen in the plaques that the phages make, if a plaque is cloudy, it is a cell that is not dead and is currently carrying phage DNA. if the p[laques are clear, it means that the phage is not temperate. having either phage does not change any process of research.
Yes, our phage is apart of the Siphoviridae based on its long tail and because it is a temperate phage. we could not determine cluster because we could not perform a restriction digest sadly
You did a really well job in this presentation! I enjoyed how you explained the relevancy and methods clearly, but I was curious as to what your hypothesis is for this research?
thank you for the kind words, My hypothesis going into this research was if temperate phages can spread their DNA without lysing, it should be the better type of phage for phage therapy because it can more effectively infect all bacteria in a human body because of how they lysogenic cycle works.
Because of the amount of rounds of purification we did to get our high titer assay, id hope that our DNA quality and quantity would’ve been high, but we sadly could not get to it because of the amount of times we had to redo dilutions for purifications.
curesymposium.org 
What is the difference between the lysogenic cycle and the lytic cycle and how would these different processes effect your research differently? (i.e. if you had used a non-temperate phage instead)
LikeLike
The lysogenic cycle and lytic cycle are 2 parts of the same life cycle for bacteriophages. the lysogenic cycle reproduces its viral DNA with the prokaryotic bacteria, meaning the bacteria continues to live on as the phage DNA is being replicated. the lytic cycle is when a phage inserts its DNA and erodes the cell metabolism and its means of replication, it then synthesizes itself inside of the bacteria and when the new phages have matured, they burst through the cell killing it, which is called lysing the cell. the differences can be seen in the plaques that the phages make, if a plaque is cloudy, it is a cell that is not dead and is currently carrying phage DNA. if the p[laques are clear, it means that the phage is not temperate. having either phage does not change any process of research.
LikeLike
Do you have any predictions as to what family of phage “bruh” is in or what cluster it may be a a part of?
LikeLike
Yes, our phage is apart of the Siphoviridae based on its long tail and because it is a temperate phage. we could not determine cluster because we could not perform a restriction digest sadly
LikeLike
Do you have any predictions as to what family your phage might be in or what cluster it could be a part of?
LikeLike
You did a really well job in this presentation! I enjoyed how you explained the relevancy and methods clearly, but I was curious as to what your hypothesis is for this research?
LikeLike
thank you for the kind words, My hypothesis going into this research was if temperate phages can spread their DNA without lysing, it should be the better type of phage for phage therapy because it can more effectively infect all bacteria in a human body because of how they lysogenic cycle works.
LikeLike
If you were to do DNA isolation how do you think your quality would turn out unless that wasn’t done. Otherwise good job!
LikeLike
Because of the amount of rounds of purification we did to get our high titer assay, id hope that our DNA quality and quantity would’ve been high, but we sadly could not get to it because of the amount of times we had to redo dilutions for purifications.
LikeLike