The poster is located beneath the video. My computer was recently damaged and unable to pick up audio. When submitting, I uploaded two different videos. One is the PowerPoint with no audio and the other is this one. I was unable to sync them together. Sorry about the visual aspect of the presentation.
We are able to hypothesize a cluster with DNA isolation and sequencing. DNA isolation is necessary to make sure we have good quality and quantity of DNA. Sequencing gives me a rough estimate what cluster and subcluster the bacteriophage belongs to. A PCR can then be run to confirm this hypothesis.
If it was a temperate phage, then it would be disqualified from phage therapy. This is because a temperate phage can integrate its DNA or RNA into the host genome (called the prophage). If the host bacteria in question has mutated to the point where it is resistant to most antibiotics and potentially some bacteriophages, then there is a change I am increasing its infection rate or resistance. This is because the rate of mutations increases when virulent DNA or RNA is introduced to the host genome.
My apologies again that my poster is not part of the video. When a phage bursts or lyses a cell, they release lysins and hollins, which are designed to break down cell walls or biofilms. Phages can act as an antibiotic by infection and then bursting an antibiotic resistant bacteria cell. A temperate phage is a type of virus that has the potential to incorporate its DNA or RNA into the host genome (called prophage). It was lie dormant in prophage, until a stimulus such as UV light activates its lytic cycle. It will then hijack the host bacteria’s machinery to produce several copies of itself before bursting or lysing the cell to infect more bacteria.
Unfortunately, we could not run a quality control due to the amount of time we had. A quality control is conducted after isolating DNA and determines if the DNA is high quality and quantity. High quality is a distinct band and high quantity means it is a bright band. Smearing means the DNA is degraded too much. If we had more time, we would have done the quality control first to ensure that our DNA was viable.
curesymposium.org 
Where is the poster?
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The poster is located beneath the video. My computer was recently damaged and unable to pick up audio. When submitting, I uploaded two different videos. One is the PowerPoint with no audio and the other is this one. I was unable to sync them together. Sorry about the visual aspect of the presentation.
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Why are you doing DNA isolation and sequencing? What does that mean in terms of the broader scope of your research?
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We are able to hypothesize a cluster with DNA isolation and sequencing. DNA isolation is necessary to make sure we have good quality and quantity of DNA. Sequencing gives me a rough estimate what cluster and subcluster the bacteriophage belongs to. A PCR can then be run to confirm this hypothesis.
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Good job! What would you have done differently if you had a temperate phage? Would you have changed your future directions?
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If it was a temperate phage, then it would be disqualified from phage therapy. This is because a temperate phage can integrate its DNA or RNA into the host genome (called the prophage). If the host bacteria in question has mutated to the point where it is resistant to most antibiotics and potentially some bacteriophages, then there is a change I am increasing its infection rate or resistance. This is because the rate of mutations increases when virulent DNA or RNA is introduced to the host genome.
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I am still a bit confused on how the phage can act as an antibiotic and the difference between the temperate and lytic methods of activity?
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My apologies again that my poster is not part of the video. When a phage bursts or lyses a cell, they release lysins and hollins, which are designed to break down cell walls or biofilms. Phages can act as an antibiotic by infection and then bursting an antibiotic resistant bacteria cell. A temperate phage is a type of virus that has the potential to incorporate its DNA or RNA into the host genome (called prophage). It was lie dormant in prophage, until a stimulus such as UV light activates its lytic cycle. It will then hijack the host bacteria’s machinery to produce several copies of itself before bursting or lysing the cell to infect more bacteria.
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How can you make sure the DNA you are sequencing is better quality and reduce smearing?
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Unfortunately, we could not run a quality control due to the amount of time we had. A quality control is conducted after isolating DNA and determines if the DNA is high quality and quantity. High quality is a distinct band and high quantity means it is a bright band. Smearing means the DNA is degraded too much. If we had more time, we would have done the quality control first to ensure that our DNA was viable.
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