We actually aren’t sure why we had 2 different sized plaques before doing the branch purification. We assumed it meant that we had 2 phages in our sample, but turns out it was only one with a funky morphology.
We would run a PCR using primers from other phage clusters to try to find a match since all of the primers we already tried came back with inconclusive results.
We really aren’t sure. We assumed that we had 2 phage still in the sample, but after doing as many rounds of purification as we did prior to that, that shouldn’t have happened. Then the branch purification showed that we did only have 1 phage.
curesymposium.org 
Could you go into more detail about why your phage plaques (not sure if I spelled right) were bigger than others?
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Could you go into more details as to why your phage plaques (not sure if I spelled right) were bigger than others?
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We actually aren’t sure why we had 2 different sized plaques before doing the branch purification. We assumed it meant that we had 2 phages in our sample, but turns out it was only one with a funky morphology.
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Hi, great job!! Could you explain what a HTL is?
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HTL stands for high titer lysate, and it essentially is a highly concentrated phage solution.
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HTL stands for high titer lysate, and it essentially is a highly concentrated phage solution along with the phage and flooding buffers that we used.
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how would more PCR tests help you determine the phage cluster?
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We would run a PCR using primers from other phage clusters to try to find a match since all of the primers we already tried came back with inconclusive results.
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What do you think caused the weird morphology on the 20-200 plaque plate?
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We really aren’t sure. We assumed that we had 2 phage still in the sample, but after doing as many rounds of purification as we did prior to that, that shouldn’t have happened. Then the branch purification showed that we did only have 1 phage.
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