Likely due to an error during DNA isolation. Removing the column from the syringe incorrectly the pressure disrupted the membrane which caused loss of DNA.
Scientists can isolate lytic phages that target specific bacteria in livestock and agriculture, which will effectively eliminate the bacteria from the produce. Hence eliminating the use of antibiotics.
curesymposium.org 
Why do you think no DNA bands showed up on your gel for the phage?
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Likely due to an error during DNA isolation. Removing the column from the syringe incorrectly the pressure disrupted the membrane which caused loss of DNA.
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What do you mean when you say “titer”?
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The titer is the precise number of active phage particles called plaque-forming units.
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Great Job! Why did you chose to experiment on M. smegmatis?
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Thank you!
It was assigned for us to use. Also, it is a nonpathogenic soil bacterium, and we did isolate our phage from a soil sample.
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You mentioned use of trahkary’s in realms such as agriculture and livestock. How might scientist use or experiment in terms of these areas?
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Scientists can isolate lytic phages that target specific bacteria in livestock and agriculture, which will effectively eliminate the bacteria from the produce. Hence eliminating the use of antibiotics.
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How did you hypothesize that the phage could potentially be a member of the O cluster?
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We hypothesized that our phage might be in the O cluster because it shares the same characteristics and morphology as the phage in this cluster.
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