This is a really cool poster! Is there any solution to the potential issue of horizontal gene transfer, or would it simply rule out your phage from therapeutic use?
Because my phage is temperate it would be ruled out form therapeutic use most likely, unless it was further tested and genetically modified so that it would be lytic.
Both lytic and lysogenic phages are capable of going through the lytic cycle, but temperate phages only do so about 20 percent of the time, which is why they aren’t a great option for therapy. Temperate phages arent capable of losing the cell so they integrate into the genome of the host cell and replicate with it.
If we had a lytic phage rather than temperate than the infection of the bacteria would have been much faster because the phage would have immediately lyse the bacteria and killing it. The electron microscopy image would look different as well because siphoveridae phages are temperate.
This is a really cool poster! Is there any solution to the potential issue of horizontal gene transfer, or would it simply rule out your phage from therapeutic use?
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Because my phage is temperate it would be ruled out form therapeutic use most likely, unless it was further tested and genetically modified so that it would be lytic.
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Hi, what is meant by horizontal gene transfer?
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Horizontal gene transfer is simply just when genetic information is transferred between organisms.
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Hi, why do different species of phage use different infection paths?
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Both lytic and lysogenic phages are capable of going through the lytic cycle, but temperate phages only do so about 20 percent of the time, which is why they aren’t a great option for therapy. Temperate phages arent capable of losing the cell so they integrate into the genome of the host cell and replicate with it.
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If you had a lytic, rather than a temperate phage, in this experiment how would that change your results?
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If we had a lytic phage rather than temperate than the infection of the bacteria would have been much faster because the phage would have immediately lyse the bacteria and killing it. The electron microscopy image would look different as well because siphoveridae phages are temperate.
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