The restriction Digest was run to help determine what cluster our phage was part of, which we hypothesized to be the A1 cluster. A cluster is just a grouping of similar phages based on % similarity of their genome
We know our phage targets M. Smeg bacteria for sure, and I believe it would also be effective against bacteria closely related to M. Smeg, but I don’t know for sure without further testing!
Good job! What was the purpose of the control gel? What does it measure exactly?
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The control gel measures if there is high enough quality and quantity DNA to proceed with experiments!
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What was the purpose of the restriction diet?
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The restriction Digest was run to help determine what cluster our phage was part of, which we hypothesized to be the A1 cluster. A cluster is just a grouping of similar phages based on % similarity of their genome
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What kind of bacteria do you think your phage could target?
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We know our phage targets M. Smeg bacteria for sure, and I believe it would also be effective against bacteria closely related to M. Smeg, but I don’t know for sure without further testing!
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