Through sequencing and recording our phage on phagesdb.com, scientists all across the world can see our results and possibly even test our archived phage for use cases such as phage therapy. Methods like these are useful to understand the morphology of our phage which would be beneficial to other scientists wanting to study a unique phage like ours.
Subcluster is very difficult to predict from virion morphology as most phages in the same cluster have the same average virion sizes even if they are in different subclusters. Therefore, only through genomic sequencing can we find Donathan’s subcluster.
Hello! I really enjoyed your presentation. I was wondering if you think it would be beneficial to run additional trials with different dilutions? Does any of your research into previous tests indicate any promise for this, or do you think that the trial you ran with 10^-1 dilution will be the most effective? Thanks!
Yes! More trials leads to more significant data and more concrete conclusions. We have ran tests at different dilutions that yielded less plaques after 10^-2 which is normal. The 10^-1 dilution suggested an inconsistency that can be explained by a very lytic phage in culture.
curesymposium.org 
How can your findings help other labs across the country? How can the methods you use be beneficial for them?
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Through sequencing and recording our phage on phagesdb.com, scientists all across the world can see our results and possibly even test our archived phage for use cases such as phage therapy. Methods like these are useful to understand the morphology of our phage which would be beneficial to other scientists wanting to study a unique phage like ours.
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What experimental procedure will you perform to isolate the phage DNA?
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To isolate the phage DNA, we performed two rounds of 4 1:10 serial dilutions to purify the phage. The isolation was confirmed by Electron Microscopy.
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Hey Xu, I was wondering if you had any predictions for what the subcluster of Donathan could be unless you gusy weren’t able to get there.
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Subcluster is very difficult to predict from virion morphology as most phages in the same cluster have the same average virion sizes even if they are in different subclusters. Therefore, only through genomic sequencing can we find Donathan’s subcluster.
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Hello! I really enjoyed your presentation. I was wondering if you think it would be beneficial to run additional trials with different dilutions? Does any of your research into previous tests indicate any promise for this, or do you think that the trial you ran with 10^-1 dilution will be the most effective? Thanks!
LikeLike
Yes! More trials leads to more significant data and more concrete conclusions. We have ran tests at different dilutions that yielded less plaques after 10^-2 which is normal. The 10^-1 dilution suggested an inconsistency that can be explained by a very lytic phage in culture.
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