You mentioned that you were fairly sure your phage was temperate and cited “bullseye patterns” on your agar plates as one piece of evidence. Are bullseye colony patterns typically indicative of temperate phages? Additionally, are there indicative patterns for other types of phage?
Most of your question was answered below but… There are indicative patterns for phage; Temperate: bulls-eye or opaque plaques, Virulent: clear plaques.
A bulls-eye pattern on the agar plate has a clear center and cloudy outer ring. The center indicates a clear area where the phage has lysed the host bacterium, while the cloudy outer ring shows an area where the bacterium and phage are residing in lysogeny. Only temperate phages undergo both lifecycles.
Great presentation!
You mentioned the significance of phage in your presentation but what is the significance of having a temperate phage like the one you isolated? In other words, what characteristics of the temperate phage are useful for research and treatment options?
To date, only virulent phages have been used as pathogen treatment options, but temperate phages are very important in their own way. They can be used to study genome expression and transduction. An example of this from the poster is lambda bacteriophage which targets E.coli.
Awesome poster 🙂 In the EM imagery, is the appearance of your phage typical? What do those results imply in the larger context of your experiment (are specific morphology types associated with different clusters, etc)?
The appearance of my phage does seem typical it seemed to be a 50/50 split between virulent and temperate phage among my peers. Yes, specific morphology can usually be seen amongst different clusters indicating many types of phage – usually seen early on in the experimental process where we have not fully isolated a phage species.
curesymposium.org 
You mentioned that you were fairly sure your phage was temperate and cited “bullseye patterns” on your agar plates as one piece of evidence. Are bullseye colony patterns typically indicative of temperate phages? Additionally, are there indicative patterns for other types of phage?
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Most of your question was answered below but… There are indicative patterns for phage; Temperate: bulls-eye or opaque plaques, Virulent: clear plaques.
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What is a bulls eye pattern on the agar plate?
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and what does it typically look like on a plate?
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A bulls-eye pattern on the agar plate has a clear center and cloudy outer ring. The center indicates a clear area where the phage has lysed the host bacterium, while the cloudy outer ring shows an area where the bacterium and phage are residing in lysogeny. Only temperate phages undergo both lifecycles.
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Great presentation!
You mentioned the significance of phage in your presentation but what is the significance of having a temperate phage like the one you isolated? In other words, what characteristics of the temperate phage are useful for research and treatment options?
LikeLike
To date, only virulent phages have been used as pathogen treatment options, but temperate phages are very important in their own way. They can be used to study genome expression and transduction. An example of this from the poster is lambda bacteriophage which targets E.coli.
LikeLike
Awesome poster 🙂 In the EM imagery, is the appearance of your phage typical? What do those results imply in the larger context of your experiment (are specific morphology types associated with different clusters, etc)?
LikeLike
The appearance of my phage does seem typical it seemed to be a 50/50 split between virulent and temperate phage among my peers. Yes, specific morphology can usually be seen amongst different clusters indicating many types of phage – usually seen early on in the experimental process where we have not fully isolated a phage species.
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