Great job! You explained the aims of the experiment well. Did you have a chance to visualize the phage with electron microscopy, and did that give any insights into the identity of the phage?
We sort of did, I only say that because of phage was complicated in the sense of everything went smoothly up until we did restriction digest. Our professors did say there was a possibility that we could still identify it, however, we were just gonna go back and do restriction digest again once more.
Overall it was a great presentation. Regarding the process of creating a web pattern plate, why did you need to make several of them, and what were they used for?
These were the dilution plates leading up to the web pattern plate. So this was the last one of dilution and purification process that we had before making the web pattern plate.
My phage was lytic because it matched the morphology typically seen in lytic phages. We couldn’t determine much, due to the restriction digest leading up to the microscopy results.
Great job on explaining everything! Two things. Your gel results seemed to be dragged, are those primer dimers or an issue with your GoTaq?Another thing was your phage lytic or lysogenic?
curesymposium.org 
Great job! You explained the aims of the experiment well. Did you have a chance to visualize the phage with electron microscopy, and did that give any insights into the identity of the phage?
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We sort of did, I only say that because of phage was complicated in the sense of everything went smoothly up until we did restriction digest. Our professors did say there was a possibility that we could still identify it, however, we were just gonna go back and do restriction digest again once more.
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Overall it was a great presentation. Regarding the process of creating a web pattern plate, why did you need to make several of them, and what were they used for?
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These were the dilution plates leading up to the web pattern plate. So this was the last one of dilution and purification process that we had before making the web pattern plate.
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Great presentation. Could temperate phages potentially be used in phage therapy? Could they be changed to lytic phages?
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It could since there is already research out there saying that it could be changed into a variant with the help of lytic and another variant.
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Is your phage lytic or lysogenic? Were you able to determine anything about it from your microscopy results?
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My phage was lytic because it matched the morphology typically seen in lytic phages. We couldn’t determine much, due to the restriction digest leading up to the microscopy results.
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Great job on explaining everything! Two things. Your gel results seemed to be dragged, are those primer dimers or an issue with your GoTaq?Another thing was your phage lytic or lysogenic?
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The phage was lytic and it was the primers. There were no cuts when we were experimenting with our phage.
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