Thanks, Geneva! The distinct cuts produced by each of these five enzymes in our phage DNA allowed us to compare the number of cuts countable on the gel with the number of cuts these enzymes produced in other phases of a known cluster. This allowed us to make hypotheses about which cluster and subcluster we suspected our phage to be. For example, if our phage DNA was cut around 15 times by BamHI, 5 times by ClaI etc., and there were known archived phage DNA and this matched with the number of times each enzyme cut DNA of database phages of the C1 subcluster, we might guess that our phage could be of the C1 subcluster as well.
Thank you, Sydney! These subclusters could tell us about the properties the phage may be likely to have carts in useful properties, as phages within the same cluster have a large degree of genetic similarity. For example, if it were found that phages of the C1 tended to be effective at infecting a specific pathogenic mycobacterium, we may be able to infer that another phage of this cluster could be useful for this purpose as well. Whether the phage is classified as siphoviridae, myoviridae, or podoviridae mostly gives us information about the physical structure of the phage, in this case, indicating that our phage has a long, flexible, and non-contractile tail. Being a siphoviridae phage may allow us to compare our phage with other lytic siphoviridae if they are found to be useful for therapy and may have certain trends of advantages or disadvantages, but this is a relatively wide classifier and there are phages with a wide variety of hosts, lifecycles, and lytic capabilities within it.
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Hi Jocelyn! What was the significance of determining that BamHI, Cla1, HaeIII, HindIII, and SalI produced distinct cuts on your gel experiment?
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Thanks, Geneva! The distinct cuts produced by each of these five enzymes in our phage DNA allowed us to compare the number of cuts countable on the gel with the number of cuts these enzymes produced in other phases of a known cluster. This allowed us to make hypotheses about which cluster and subcluster we suspected our phage to be. For example, if our phage DNA was cut around 15 times by BamHI, 5 times by ClaI etc., and there were known archived phage DNA and this matched with the number of times each enzyme cut DNA of database phages of the C1 subcluster, we might guess that our phage could be of the C1 subcluster as well.
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Great presentation!
What is the significance of having a phage with A2 or C1 subcluster characteristics and siphoviredae morphology?
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Thank you, Sydney! These subclusters could tell us about the properties the phage may be likely to have carts in useful properties, as phages within the same cluster have a large degree of genetic similarity. For example, if it were found that phages of the C1 tended to be effective at infecting a specific pathogenic mycobacterium, we may be able to infer that another phage of this cluster could be useful for this purpose as well. Whether the phage is classified as siphoviridae, myoviridae, or podoviridae mostly gives us information about the physical structure of the phage, in this case, indicating that our phage has a long, flexible, and non-contractile tail. Being a siphoviridae phage may allow us to compare our phage with other lytic siphoviridae if they are found to be useful for therapy and may have certain trends of advantages or disadvantages, but this is a relatively wide classifier and there are phages with a wide variety of hosts, lifecycles, and lytic capabilities within it.
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