“Lytic” means that it follows the lytic life cycle, where the viral genome is immediately used to begin producing new viruses instead of being integrated into the host cell’s genome. The application is that this phage will be more efficient at killing bacteria and will not have the risk of transferring unwanted genes in the process.
We used our restriction digest results to classify Auspice into cluster A as our PCR results came back inconclusive for when we tested different subclusters within cluster A.
Hello, good presentation overall! 🙂 For my own curiosity, are there external factors or things within the soil that could affect the sample collected?
Things like temperature, soil composition, and different types of soil bacteria could affect the sample collected and what phages would be present in it.
Great presentation! How do you think the location of the dirt sample would effect your outcome, and what are some places you think would yield the best phages?
The location of the soil sample will influence what bacteria are present in the soil, which will then affect what phages will be present and the quantities of them. We collected our sample from underneath a tree (in shade) from damp soil after rain, so I believe these conditions were ideal for yielding efficient phages.
The location of the soil sample will influence what bacteria are present in the soil, which will then affect what phages will be present and the quantities of them. We collected our sample from underneath a tree (in shade) from damp soil after rain, so I believe these conditions were ideal for yielding efficient phages.
curesymposium.org 
What does it mean that it’s lytic? What is the application of this information?
LikeLike
“Lytic” means that it follows the lytic life cycle, where the viral genome is immediately used to begin producing new viruses instead of being integrated into the host cell’s genome. The application is that this phage will be more efficient at killing bacteria and will not have the risk of transferring unwanted genes in the process.
LikeLike
Good presentation! Were you able to classify “Auspice” in cluster A because of the restriction digests, the PCR, or both?
LikeLike
We used our restriction digest results to classify Auspice into cluster A as our PCR results came back inconclusive for when we tested different subclusters within cluster A.
LikeLike
Nice presentation! Were you able to classify “Auspice” as cluster A because of the restriction digest, the PCR, or both?
LikeLike
Hello, good presentation overall! 🙂 For my own curiosity, are there external factors or things within the soil that could affect the sample collected?
LikeLike
Things like temperature, soil composition, and different types of soil bacteria could affect the sample collected and what phages would be present in it.
LikeLike
Great presentation! How do you think the location of the dirt sample would effect your outcome, and what are some places you think would yield the best phages?
LikeLike
The location of the soil sample will influence what bacteria are present in the soil, which will then affect what phages will be present and the quantities of them. We collected our sample from underneath a tree (in shade) from damp soil after rain, so I believe these conditions were ideal for yielding efficient phages.
LikeLike
The location of the soil sample will influence what bacteria are present in the soil, which will then affect what phages will be present and the quantities of them. We collected our sample from underneath a tree (in shade) from damp soil after rain, so I believe these conditions were ideal for yielding efficient phages.
LikeLike