Good job on your presentation! One question I have is what was the purpose behind the spot titer assay? From my understanding, the purification of the lytic phage shown in your figure one confirmed your identification of it as a lytic phage, but what were your goals behind the spot titer assay?
The first spot titer test is to confirm that we had plaques present, we only had two spots on our first test and they were from one sample of our enrichment plate. The second one was to show the dilutions of the high titer lysate and get to a dilution with a countable number of plaques. Hope this answers your question!
Once our phage genome is sequenced it will be put into a database that has every single phage discovered in it, as well as ~3mL of our phage lysate, sent to an institution to be stored. Further research with the sequence will allow us to know if our phage is novel (undiscovered) or common. With us having the known genome, we can help aid in phage therapy. Essentially, we are just adding phages into the database so that if one with that specific genome is needed it is available.
curesymposium.org 
Good job on your presentation! One question I have is what was the purpose behind the spot titer assay? From my understanding, the purification of the lytic phage shown in your figure one confirmed your identification of it as a lytic phage, but what were your goals behind the spot titer assay?
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The first spot titer test is to confirm that we had plaques present, we only had two spots on our first test and they were from one sample of our enrichment plate. The second one was to show the dilutions of the high titer lysate and get to a dilution with a countable number of plaques. Hope this answers your question!
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How can your results be helpful for other labs across the country?
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Once our phage genome is sequenced it will be put into a database that has every single phage discovered in it, as well as ~3mL of our phage lysate, sent to an institution to be stored. Further research with the sequence will allow us to know if our phage is novel (undiscovered) or common. With us having the known genome, we can help aid in phage therapy. Essentially, we are just adding phages into the database so that if one with that specific genome is needed it is available.
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Why do you think that the PCR result would have shown?
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PCR results would confirm our cluster and subcluster
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