Our phage was isolated from the environment, so it was random chance. On our enrichment plaque assay, which had multiple species of phage present, and there was no reason for choosing the plaque that we did.
If I understood it right, a temperate phage is less efficient at killing the host bacteria? Do you know how one might turn a temperate phage into a lytic phage?
You would be correct. Genetic modification is needed for turning a temperate phage into a lytic phage. This would mean removing the suppressor protein from a temperate phage (which is responsible for switching between the lytic and lysogenic cycle) and removing the integrase enzyme. (responsible for integrating the viral DNA into the bacterial DNA) By removing both of these proteins the phage should be forced into the lytic lifecycle.
curesymposium.org 
Why did you pick the phage you focused on in the research?
LikeLike
Our phage was isolated from the environment, so it was random chance. On our enrichment plaque assay, which had multiple species of phage present, and there was no reason for choosing the plaque that we did.
LikeLike
If I understood it right, a temperate phage is less efficient at killing the host bacteria? Do you know how one might turn a temperate phage into a lytic phage?
LikeLike
You would be correct. Genetic modification is needed for turning a temperate phage into a lytic phage. This would mean removing the suppressor protein from a temperate phage (which is responsible for switching between the lytic and lysogenic cycle) and removing the integrase enzyme. (responsible for integrating the viral DNA into the bacterial DNA) By removing both of these proteins the phage should be forced into the lytic lifecycle.
LikeLike