I really liked how you enlarged the results when you talked about them, it was a lot easier to see them that way. Also your figures looked really nice and were easy to interpret. How do you think your phage was contaminated?
It is still unknown how our phage was contaminated. It could have been an airborne phage but that theory has not been confirmed due to the fact that a few groups did not have the contaminant in their sample.
In future experiments how would propose to isolate the contaminant phage from the rest of the phage? Also if the contaminant phage isn’t part of the O-cluster, what would that mean for the experiment?
In order to isolate the contaminant we would need to preform multiple serial dilutions until we were able to isolate it from the other phage present. Through DNA sequencing is how we can confirm or deny if the phage is a part of the O-cluster. If it isn’t then we can see from its DNA what cluster it is in. We also can continue to run cluster identification tests to determine the cluster once the phage is isolated.
I really liked how you enlarged the results when you talked about them, it was a lot easier to see them that way. Also your figures looked really nice and were easy to interpret. How do you think your phage was contaminated?
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It is still unknown how our phage was contaminated. It could have been an airborne phage but that theory has not been confirmed due to the fact that a few groups did not have the contaminant in their sample.
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What does it mean to be a siphoviridae phage?
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A siphoviridae phage has an icosahedral head and a long non-contractile tail. The tail is longer than the head of the phage making it a siphoviridae!
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In future experiments how would propose to isolate the contaminant phage from the rest of the phage? Also if the contaminant phage isn’t part of the O-cluster, what would that mean for the experiment?
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In order to isolate the contaminant we would need to preform multiple serial dilutions until we were able to isolate it from the other phage present. Through DNA sequencing is how we can confirm or deny if the phage is a part of the O-cluster. If it isn’t then we can see from its DNA what cluster it is in. We also can continue to run cluster identification tests to determine the cluster once the phage is isolated.
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