Great job to both of you! Were you guys ever able to determine where your DNA became damaged during the duration of your lab? This would be super beneficial for anyone to know who wants to repeat your experiment(s)! 🙂
We weren’t able to determine where the DNA became damaged since we went straight into restriction digest and we didn’t have time to redo our DNA purification.
I think the fact that we were only able to do DNA purification once affected our results because we didn’t know if our purification had worked and based off of our gel results the purification didn’t work as well as we would have liked.
We ran our gel for about 20-30 minutes and this was determined by our lab manual. We stopped running the gel once the bromophenol blue was about 80% down the gel.
There have been some studies where temperate phages have been genetically engineered, so I think our phage will be able to be used in future phage research.
curesymposium.org 
Great job to both of you! Were you guys ever able to determine where your DNA became damaged during the duration of your lab? This would be super beneficial for anyone to know who wants to repeat your experiment(s)! 🙂
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We weren’t able to determine where the DNA became damaged since we went straight into restriction digest and we didn’t have time to redo our DNA purification.
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Good Job! How do you think the fact that you were only able to do the phage DNA isolation and the restriction digest affected your results?
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I think the fact that we were only able to do DNA purification once affected our results because we didn’t know if our purification had worked and based off of our gel results the purification didn’t work as well as we would have liked.
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How long did you run the gel for? Why did you choose to run it for that amount of tie initially?
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We ran our gel for about 20-30 minutes and this was determined by our lab manual. We stopped running the gel once the bromophenol blue was about 80% down the gel.
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Great job! How do/do you think your temperate phage, specifically, can be used in phage research/therapy?
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There have been some studies where temperate phages have been genetically engineered, so I think our phage will be able to be used in future phage research.
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This is a reply to the question above
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This is a reply to the question above.
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