I think you did a great job at presenting your research to a diverse audience. I think overall your presentation was very precise and clear and that you did a great job at covering the foundations of your research. Great job!
Thank you so much for your nice comments! One possible experimental error that affected our research was an error during our purifications. That means that we didn’t purify our phage enough.
Excellent presentation, but could you possibly explain what exactly a restriction digest is? It was mentioned several times throughout the presentation yet its significance was not really explained.
Thank you! Yes, I definitely can. Restriction digest is a way to get a prediction of what cluster our phage is in based on the amounts of cuts each enzyme made to our phages DNA.
Hi, I performed the restriction digest by placing our DNA in each of the gel slots on the top along with one enzyme in each slot. The first slot was our ladder DNA with no enzyme. We isolated the DNA through a series of steps. A brief explanation is we used DNA wizards( small plastic pieces) to catch the DNA and rinsed it multiple times with isopropanol and DI water.
I think you did a great job at presenting your research to a diverse audience. I think overall your presentation was very precise and clear and that you did a great job at covering the foundations of your research. Great job!
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Based on your conclusions and findings, what possible experimental errors do you think could have affected your research?
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Thank you so much for your nice comments! One possible experimental error that affected our research was an error during our purifications. That means that we didn’t purify our phage enough.
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Excellent presentation, but could you possibly explain what exactly a restriction digest is? It was mentioned several times throughout the presentation yet its significance was not really explained.
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Thank you! Yes, I definitely can. Restriction digest is a way to get a prediction of what cluster our phage is in based on the amounts of cuts each enzyme made to our phages DNA.
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How did you perform the restriction digest, and how did you isolate the DNA? Great job presenting!
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Hi, I performed the restriction digest by placing our DNA in each of the gel slots on the top along with one enzyme in each slot. The first slot was our ladder DNA with no enzyme. We isolated the DNA through a series of steps. A brief explanation is we used DNA wizards( small plastic pieces) to catch the DNA and rinsed it multiple times with isopropanol and DI water.
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What do these subclusters mean?
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Great question! The subclusters are just groups of more closely related phage!
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