Hi thanks for the quesiton, a titer is a value which represents the total pfu (plaque forming units) per ml. A halo is a lightly shaded area around the plaque within the bacterial lawn.
Since you determined that your phage is lytic, would this be advantageous if you were to use it for phage therapy? Is it possible to change a temperate phage so it only undergoes the lytic lifecycle?
i dont know if my comment glitched, so im gonna be submitting my question again. Sorry if this is a duplicate!
Since you determined that your phage is lytic, would this be advantageous if you were to use it for phage therapy? Is it possible to change a temperate phage to only undergo the lytic life cycle?
Does plaque size matter when it comes to finding a phage? What is the “ideal” phage and do you think there is a certain place (dirt) you can find it in?
Hi thanks for your question! I find that warmer, moist soil is best to find phage. There is no ideal phage, but rather one that could infect bacterial infections which affect humans. Plaque size is not important, but helps with determining morphology.
curesymposium.org 
Hi, Muschinski. Excellent presentation! Can you explain to me what a titer is? Also, what does it mean when a bacteria has a halo?
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Hi thanks for the quesiton, a titer is a value which represents the total pfu (plaque forming units) per ml. A halo is a lightly shaded area around the plaque within the bacterial lawn.
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Hi Peter! Great work 🙂
Since you determined that your phage is lytic, would this be advantageous if you were to use it for phage therapy? Is it possible to change a temperate phage so it only undergoes the lytic lifecycle?
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Thanks. Yes, it is possible to change from lytic to lysogenic by isolated and removing certain proteins within the phage.
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Hi Peter! Great work 🙂
i dont know if my comment glitched, so im gonna be submitting my question again. Sorry if this is a duplicate!
Since you determined that your phage is lytic, would this be advantageous if you were to use it for phage therapy? Is it possible to change a temperate phage to only undergo the lytic life cycle?
LikeLike
Hi Peter, great presentation! Does the kind of phage or where you collected it from affect the efficacy of a potential phage treatment?
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As far as I know where you collected the phage does not. Whether the phage is lytic or lysogenic does have an effect.
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Hi Peter!
Does plaque size matter when it comes to finding a phage? What is the “ideal” phage and do you think there is a certain place (dirt) you can find it in?
LikeLike
Hi thanks for your question! I find that warmer, moist soil is best to find phage. There is no ideal phage, but rather one that could infect bacterial infections which affect humans. Plaque size is not important, but helps with determining morphology.
LikeLike