The life cycles are not necessarily important to the experiment itself, but very necessary when archiving our phage data. The goal of isolating these phage are to create a database that scientists experimenting with phage therapy (an alternate possibility to antibiotics) can use. The reason it is important to know the life cycle is because lytic phages will kill the host cell.
While the life cycles are not necessarily important to the experiment itself, they are important when archiving the phage and entering it into the database. Archiving the phages aims to create a database that other scientists researching phage therapy (an alternate possibility to antibiotics) can use to find all the data on a specific phage.
If I had more time to run this experiment, I would take the time to ensure that the lysate is completely purified and then continue with the rest of the experiments. I would get a chance to run a PCR EM, calculate the concentration of the High Titer Lysate, and fully archive my data.
Thank you! The big picture is to create a database on phages that scientists researching phage therapy (an alternate possibility to antibiotics) can use to find information on a specific phage.
Thank you! I think I would try to finish my experiments (without having to rush them) on this one first. But I would definitely jump at the opportunity to do it again on a different phage as well!
We had no clue what to name it so we looked up a random word generator and thought “PrettyUseless” was very fitting. We hadn’t fully purified the phage because we were told to just continue the experiments so we had some results to present. This meant that whatever data we collected could be used for any future experiment, aka the data was pretty useless.
So like we said it is a temperate phage (I.e. can go through lytic and lysogenic cycles depending on the evironment). What this means is that it has the possibility to kill the host cell so if the phage is used for phage therapy, it would need to be genetically modified so that it only goes through the lysogenic cycle (which will not kill the cell).
curesymposium.org 
How are the cycles relevant to the experiment that you performed?
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The life cycles are not necessarily important to the experiment itself, but very necessary when archiving our phage data. The goal of isolating these phage are to create a database that scientists experimenting with phage therapy (an alternate possibility to antibiotics) can use. The reason it is important to know the life cycle is because lytic phages will kill the host cell.
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While the life cycles are not necessarily important to the experiment itself, they are important when archiving the phage and entering it into the database. Archiving the phages aims to create a database that other scientists researching phage therapy (an alternate possibility to antibiotics) can use to find all the data on a specific phage.
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If you have more time, how would you redo or improve your experiment?
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If I had more time to run this experiment, I would take the time to ensure that the lysate is completely purified and then continue with the rest of the experiments. I would get a chance to run a PCR EM, calculate the concentration of the High Titer Lysate, and fully archive my data.
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Hey there! Good job at presenting and explaining your figures! I wanted to know that the “big picture” was of your research.
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Thank you! The big picture is to create a database on phages that scientists researching phage therapy (an alternate possibility to antibiotics) can use to find information on a specific phage.
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I really enjoyed listening to your presentation! I’m curious if you would try to sequence different phages if given more time?
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Thank you! I think I would try to finish my experiments (without having to rush them) on this one first. But I would definitely jump at the opportunity to do it again on a different phage as well!
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Is there a story behind the name “PrettyUseless”?
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We had no clue what to name it so we looked up a random word generator and thought “PrettyUseless” was very fitting. We hadn’t fully purified the phage because we were told to just continue the experiments so we had some results to present. This meant that whatever data we collected could be used for any future experiment, aka the data was pretty useless.
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Why does this phage having two different cycles matter?
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So like we said it is a temperate phage (I.e. can go through lytic and lysogenic cycles depending on the evironment). What this means is that it has the possibility to kill the host cell so if the phage is used for phage therapy, it would need to be genetically modified so that it only goes through the lysogenic cycle (which will not kill the cell).
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