If the PCR was successful there would be DNA cuts within the lanes containing our DNA and the primers chosen, but since there are no visible cuts in those lanes the PCR was inconclusive.
I read a journal article (Ni 2021 which is in the references if you want to check it out), but they actually tested their phage and its functionality in various temperatures and pHs. For any organism environment is a huge part of survival, so say theoretically, for example if there is an antibiotic resistant bacteria in Arizona that infects and kills cacti you want a phage that can withstand high temperatures in order to survive and reproduce to finish killing off these bacteria.
Like any functional, multiplying organism its environment is extremely important for its survival. So for example if you have an acidic fruit that is being plagued by an antibiotic resistant bacteria you need to make sure that the phage can survive in acidic pH otherwise the phage would not be able to survive, multiply, and function the way it needs to. This is the same with temperature, since for example, if a bacteria induces a fever the human body can climb to over 100°F, and if we want to consider phage therapy for this patient the phage must be able to withstand high temperatures in order to survive and function the way we want.
Why do you think that your results from figure 5 showed that the phage was either in clusters a1 or b1 if your results in figure 6 showed that the phage was in neither of those cluster?
Figure 5 was an agarose gel we ran to see which enzymes cut our DNA, and based on that and the number of cuts we put our results into a website that returned back potential primers that could bind to our DNA. We picked the top two and ran a PCR with primers A1 and B1 to confirm whether or not the phage is part of cluster A or B. The primers did not cut the DNA in the PCR test, so we concluded it didn’t belong in either cluster, so our final results were inconclusive.
Great presentation! For ‘investigating conditions under which phages can survive/function’ in the future directions, what specific experiments do you want to be done to accomplish this or what experiments do you think will be helpful in finding out the answer?
I would observe the phages activity in various pHs and temperatures and measure the activity and reproduction within the environment. This will help further classify the phages and pair them up with possible bacteria based on the environment the bacteria and phage thrive in.
curesymposium.org 
Why was figure 6 inconclusive?
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If the PCR was successful there would be DNA cuts within the lanes containing our DNA and the primers chosen, but since there are no visible cuts in those lanes the PCR was inconclusive.
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This is really interesting! How do you think that the environment phage survives in could play into their therapeutic use?
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I read a journal article (Ni 2021 which is in the references if you want to check it out), but they actually tested their phage and its functionality in various temperatures and pHs. For any organism environment is a huge part of survival, so say theoretically, for example if there is an antibiotic resistant bacteria in Arizona that infects and kills cacti you want a phage that can withstand high temperatures in order to survive and reproduce to finish killing off these bacteria.
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Why do you think the pH or temperature would affect the survival?
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Like any functional, multiplying organism its environment is extremely important for its survival. So for example if you have an acidic fruit that is being plagued by an antibiotic resistant bacteria you need to make sure that the phage can survive in acidic pH otherwise the phage would not be able to survive, multiply, and function the way it needs to. This is the same with temperature, since for example, if a bacteria induces a fever the human body can climb to over 100°F, and if we want to consider phage therapy for this patient the phage must be able to withstand high temperatures in order to survive and function the way we want.
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Why do you think that your results from figure 5 showed that the phage was either in clusters a1 or b1 if your results in figure 6 showed that the phage was in neither of those cluster?
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Figure 5 was an agarose gel we ran to see which enzymes cut our DNA, and based on that and the number of cuts we put our results into a website that returned back potential primers that could bind to our DNA. We picked the top two and ran a PCR with primers A1 and B1 to confirm whether or not the phage is part of cluster A or B. The primers did not cut the DNA in the PCR test, so we concluded it didn’t belong in either cluster, so our final results were inconclusive.
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Great presentation! For ‘investigating conditions under which phages can survive/function’ in the future directions, what specific experiments do you want to be done to accomplish this or what experiments do you think will be helpful in finding out the answer?
LikeLike
I would observe the phages activity in various pHs and temperatures and measure the activity and reproduction within the environment. This will help further classify the phages and pair them up with possible bacteria based on the environment the bacteria and phage thrive in.
LikeLike