Pickleman is going into a phage archive. It can be used as a component in phage cocktails. Since it is temperate, it would need to be genetically modified to lyse bacteria before being used in phage therapy.
You did carry out PCR, but because the DNA was degraded you couldn’t determine the cluster of the phage? Do you know of a way to possibly treat DNA degradation to make it usable in PCR and other experiments?
The DNA degradation led to the hypothesis of the Phage enzyme tool analysis to be false. While the PCR worked the hypothesized cluster was not true. Another PCR could be done testing different clusters so see if a cluster can be determined.
Phage therapy is an experimental medical procedure in which a mixture of phages are being used to lyse bacteria specifically in diseases with antibiotic resistant bacteria. It is not currently FDA approved for everyday use but can be used in certain emergent cases.
The sample is purified using several rounds of 1-10 serial dilutions. The most isolated plaques were selected from the most isolated dilutions to reduce the chances of multiple phages being present in the plaque in order to create a lysate.
curesymposium.org 
What future impacts could Pickleman have for the scientific world?
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Pickleman is going into a phage archive. It can be used as a component in phage cocktails. Since it is temperate, it would need to be genetically modified to lyse bacteria before being used in phage therapy.
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You did carry out PCR, but because the DNA was degraded you couldn’t determine the cluster of the phage? Do you know of a way to possibly treat DNA degradation to make it usable in PCR and other experiments?
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The DNA degradation led to the hypothesis of the Phage enzyme tool analysis to be false. While the PCR worked the hypothesized cluster was not true. Another PCR could be done testing different clusters so see if a cluster can be determined.
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What is phage therapy?
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Phage therapy is an experimental medical procedure in which a mixture of phages are being used to lyse bacteria specifically in diseases with antibiotic resistant bacteria. It is not currently FDA approved for everyday use but can be used in certain emergent cases.
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How is the sample purified to contain only one kind of bacteriophage?
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The sample is purified using several rounds of 1-10 serial dilutions. The most isolated plaques were selected from the most isolated dilutions to reduce the chances of multiple phages being present in the plaque in order to create a lysate.
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