Great job on your presentation! I’ve understood that lytic phages can be very useful, but are there any uses for temperate phages as is (without genetic modification)?
Thanks for commenting!!! Another use of temperate phages is tagging bacteria. A type of dye can be used on the phage and the phage could be spread to places to see if bacteria is present. It would have to be very specific like detecting tuberculosis in someones body by using a phage that is known to infect tuberculosis and then adding the dye and see if it glows.
Thanks for commenting!!! Phage wont necessarily prevent multi resistant bacterial infections but it could be a way to kill a bacterial infection. Bacteria could become resistant to phages but the phage can also evolve to be able to infect a different way!!!
Thanks for commenting!!! For the restriction digest (not shown on poster), it helped determine what possible cluster our phage belonged to. So we inputted the amount of cuts done by each enzyme given to us in a program online and it searched what phage cluster our phage most likely would be in. After that to even more solidify the hypothesis we ran a PCR with primers from the clusters. In our case it was either A1 or B1. By looking at the gel and if a band was present or not we could determine what cluster our phage might be in.
Great presentation and great reasoning in your explanations! To make your phage useful in phage therapy, do you think that you would have to remove lytic repressor entirely or could you repress the repressor gene using like mRNA or something?
Thanks for commenting!!! To modify the phage, the repressor gene and a protein called integrase have to be removed. Just to clarify we want the phage to be lytic so that it can kill bacteria and by removing the repressor it inhibits the phages ability to not go into the lytic cycle. And for integrase which starts the process of DNA integration, it would not integrate its DNA meaning it would undergo the lytic cycle.
curesymposium.org 
Great job on your presentation! I’ve understood that lytic phages can be very useful, but are there any uses for temperate phages as is (without genetic modification)?
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Thanks for commenting!!! Another use of temperate phages is tagging bacteria. A type of dye can be used on the phage and the phage could be spread to places to see if bacteria is present. It would have to be very specific like detecting tuberculosis in someones body by using a phage that is known to infect tuberculosis and then adding the dye and see if it glows.
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How can phage therapy prevent multi-resistant bacterial infections? Why can’t bacteria become resistant to phages?
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Thanks for commenting!!! Phage wont necessarily prevent multi resistant bacterial infections but it could be a way to kill a bacterial infection. Bacteria could become resistant to phages but the phage can also evolve to be able to infect a different way!!!
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What do the gels show for your results exactly?
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Thanks for commenting!!! For the restriction digest (not shown on poster), it helped determine what possible cluster our phage belonged to. So we inputted the amount of cuts done by each enzyme given to us in a program online and it searched what phage cluster our phage most likely would be in. After that to even more solidify the hypothesis we ran a PCR with primers from the clusters. In our case it was either A1 or B1. By looking at the gel and if a band was present or not we could determine what cluster our phage might be in.
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Great presentation and great reasoning in your explanations! To make your phage useful in phage therapy, do you think that you would have to remove lytic repressor entirely or could you repress the repressor gene using like mRNA or something?
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Thanks for commenting!!! To modify the phage, the repressor gene and a protein called integrase have to be removed. Just to clarify we want the phage to be lytic so that it can kill bacteria and by removing the repressor it inhibits the phages ability to not go into the lytic cycle. And for integrase which starts the process of DNA integration, it would not integrate its DNA meaning it would undergo the lytic cycle.
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