Were any of your observable outcomes wildly unexpected? Were there any readings that you weren’t able to include in your poster because they weren’t useful?
All of the observed outcomes were as expected. We never had any contamination or other outliers, so all in all the purification process as well as DNA isolation went as expected.
All results were as expected, meaning we never had contamination or other outliers to work around. Since we had steady results, we did not need to include papers that would account for outliers in that way.
The EM photograph is where we really were able to see the structure of the bacteriophage (siphoviridae). This was not performed in the lab but rather sent off to the facility. Uranyl acetate and sterilized water were used on the sample once the high titer lysate was added to a small grid. This prepped it for the EM photographing process.
The siphoviridae structure is another characteristic to reference when considering possible clusters for the phage. Different clusters exhibit different morphologies, and this physical structure allows them to infect specific host bacteria.
Were any of your observable outcomes wildly unexpected? Were there any readings that you weren’t able to include in your poster because they weren’t useful?
LikeLike
All of the observed outcomes were as expected. We never had any contamination or other outliers, so all in all the purification process as well as DNA isolation went as expected.
LikeLike
Were there any observable outcomes that were extremely unexpected? Was there any reading that was not included in this poster report because of this?
LikeLike
All results were as expected, meaning we never had contamination or other outliers to work around. Since we had steady results, we did not need to include papers that would account for outliers in that way.
LikeLike
Can you elaborate a little more on the methods and how they showed you the structure of the phage?
LikeLike
The EM photograph is where we really were able to see the structure of the bacteriophage (siphoviridae). This was not performed in the lab but rather sent off to the facility. Uranyl acetate and sterilized water were used on the sample once the high titer lysate was added to a small grid. This prepped it for the EM photographing process.
LikeLike
Is there any significance to the siphoviridae morphology? Does it reveal anything about the other characteristics of the phage?
LikeLike
The siphoviridae structure is another characteristic to reference when considering possible clusters for the phage. Different clusters exhibit different morphologies, and this physical structure allows them to infect specific host bacteria.
LikeLike