Great work, Sinesio! You mentioned that you got the soil sample used in this study from CU Boulder. Where on campus did you get it from? just curious 🙂
Great presentation! It is always interesting to learn more about bacteriophages as they are a crucial part of our environment.
In your results you mentioned a high HTL number in figures 3 and 4. What exactly is an HTL and why does this number being high effect your results? Did you expect this to be high or did it differ from your predictions?
HTL stands for high titer lysate, and it is a metric to measure the density of phage in a solution. For the purpose of these experiments having a high titer is useful in ensuring that enough phage is present for the next experiment
Hi! You talked about how phage can be used in therapy settings. Do you know if your specific type of phage would be a good candidate for phage therapy?
Potentially, the main hurdle would be weather or not our phage is capable of infecting pathogenic bacteria, which would require additional testing and access to a lab cable of working with pathogens
Without sequencing it would be hard to know, but given that our restriction digest came back inconclusive twice with no obvious errors in procedure there is a chance that our phage isn’t similar to any of the main clusters
curesymposium.org 
Great work, Sinesio! You mentioned that you got the soil sample used in this study from CU Boulder. Where on campus did you get it from? just curious 🙂
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The soil sample was collected outside the Carlson gym.
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You mention the bacteriophage as torpedo, did you and your lab partner name it yourself or is this a specific type of phage?
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Torpedo was the name for the phage me and my partner came up with
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Great presentation! It is always interesting to learn more about bacteriophages as they are a crucial part of our environment.
In your results you mentioned a high HTL number in figures 3 and 4. What exactly is an HTL and why does this number being high effect your results? Did you expect this to be high or did it differ from your predictions?
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HTL stands for high titer lysate, and it is a metric to measure the density of phage in a solution. For the purpose of these experiments having a high titer is useful in ensuring that enough phage is present for the next experiment
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Hi! You talked about how phage can be used in therapy settings. Do you know if your specific type of phage would be a good candidate for phage therapy?
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Potentially, the main hurdle would be weather or not our phage is capable of infecting pathogenic bacteria, which would require additional testing and access to a lab cable of working with pathogens
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How alike to other known phage sequences do you think your phage is?
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Without sequencing it would be hard to know, but given that our restriction digest came back inconclusive twice with no obvious errors in procedure there is a chance that our phage isn’t similar to any of the main clusters
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Since both restriction digests didn’t place our phages definitively in any clusters, it is possible our phage is somewhat favorite
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