The process for discovering and purifying is the same for all phages. What you do is take a dirt sample and go through a series of experiments in order to obtain one type of phage and then test it to see what kind of viruses it is able to kill. Their isn’t a specific process that each phage goes through all of the experiments we do are the same. The only part that is different is when you get to PCR you have to test it against different clusters in order to determine what kind of phage it is therefore telling you what kind of viruses it can infect. However my partner and I were unable to get to that part of the experiment.
Lytic or lysogenic describes the morphology of the phage or what it looks like, it also determines if the phage will go through the lytic or lysogenic lifecycle. There is no real benefit to using one or the other since you aren’t able to choose the type of phage you get. For phage therapy it uses a cocktail of different kinds of phage that is specialized to the person being treated based off of what they need help with, so their very well could be both lytic and lysogenic phage used during phage therapy. To sum it up their isn’t a benefit to using one over the other.
One of the biggest limitations of the experiment was time. My partner and I were unable to finish the experiments. Another limitation is that our sample didn’t have high quality or quantity DNA which also doesn’t allow us to move on with future experiments and this can be due to our sample sitting for too long and the DNA just went away or because of improper storage.
curesymposium.org 
for your specific virus, what was the process for discovering it and purifying it from its surroundings?
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The process for discovering and purifying is the same for all phages. What you do is take a dirt sample and go through a series of experiments in order to obtain one type of phage and then test it to see what kind of viruses it is able to kill. Their isn’t a specific process that each phage goes through all of the experiments we do are the same. The only part that is different is when you get to PCR you have to test it against different clusters in order to determine what kind of phage it is therefore telling you what kind of viruses it can infect. However my partner and I were unable to get to that part of the experiment.
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Hi Mylie! Is there a benefit to using lytic or lysogenic phage in phage therapy?
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Lytic or lysogenic describes the morphology of the phage or what it looks like, it also determines if the phage will go through the lytic or lysogenic lifecycle. There is no real benefit to using one or the other since you aren’t able to choose the type of phage you get. For phage therapy it uses a cocktail of different kinds of phage that is specialized to the person being treated based off of what they need help with, so their very well could be both lytic and lysogenic phage used during phage therapy. To sum it up their isn’t a benefit to using one over the other.
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What were the limitations of this experiment?
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One of the biggest limitations of the experiment was time. My partner and I were unable to finish the experiments. Another limitation is that our sample didn’t have high quality or quantity DNA which also doesn’t allow us to move on with future experiments and this can be due to our sample sitting for too long and the DNA just went away or because of improper storage.
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