Thank you! the limitations of our research experiment are mostly human error. the lab and experiment are very well controlled, however there is lots of room for error. In multiple of our spot tests, our negative control from phage buffer was contaminated with phage. Which is a perfect example of us not being careful with our our pipette tips. We also believe our Restriction digest smearing was due to human error.
The downsides to the abundance of phages is that there are so many out there to be discovered, with all different uses. While a scientist is making a concauction of different phages to hopefully treat someone, they have so many to choose from, and maybe ones that could be. sued that aren’t discovered yet. The good news is the more we find the more patients we are able to treat.
The cause of the inconclusiveness could have been multiple things including DNA isolation not being preformed correctly, or the enzymes not being mixed correctly. It could have also been the pipetting of the DNA and enzymes in the wells of electrophoresis. It is unsure, but more than likely, it was human error. We do know for sure we had a High Titer, so we do not believe it was due to our actual DNA.
curesymposium.org 
What were the limitations in this experiment?
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Overall, really nice job!! 🙂
what were the limitations of this experiment?
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Thank you! the limitations of our research experiment are mostly human error. the lab and experiment are very well controlled, however there is lots of room for error. In multiple of our spot tests, our negative control from phage buffer was contaminated with phage. Which is a perfect example of us not being careful with our our pipette tips. We also believe our Restriction digest smearing was due to human error.
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What are some of the potential downsides to the abundance of different phages?
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The downsides to the abundance of phages is that there are so many out there to be discovered, with all different uses. While a scientist is making a concauction of different phages to hopefully treat someone, they have so many to choose from, and maybe ones that could be. sued that aren’t discovered yet. The good news is the more we find the more patients we are able to treat.
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What does the web pattern on the plate mean?
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The web pattern plate shows substantial growth from our phage and it was able to create a High Titer Lysate of one purified phage.
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What may have been the cause of the inconclusiveness or smearing for the experiment done in figure 4?
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The cause of the inconclusiveness could have been multiple things including DNA isolation not being preformed correctly, or the enzymes not being mixed correctly. It could have also been the pipetting of the DNA and enzymes in the wells of electrophoresis. It is unsure, but more than likely, it was human error. We do know for sure we had a High Titer, so we do not believe it was due to our actual DNA.
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