No, not yet. We would need to look at our restriction digest results again and compare those results again with already classified phages restriction digest results in order to make new predictions and then test those predictions on a PCR again.
Hi! I thought your presentation was really good! I am wondering if there was a reason why you plan to run another PCR in the future instead of for this presentation. Going off of that, in what ways do you think you will modify your PCR if you were to do this again and how would that affect your results?
We would run another PCR in order to hopefully find out what cluster our phage is in for sure. In the future we would modify our PCR by testing different clusters with our phages DNA in order to see if we get a positive result for one of those other clusters
Ou negative control is everything in the PCR mixture except for our predicted clusters (so our phage DNA and PCR reaction mix) and this should show nothing in our results. Our positive control is already classified phage DNA with its cluster (we used phage in the A4 cluster with A4 cluster primer mix) and this should show one band to show that it is correct.
It would help researchers find out what specific bacteria our phage is designed to attack and that would then help us know what bacterial infection our phage could be used for to treat in phage therapy.
Hi! You mentioned something about ‘blast analysis’ at the end of your presentation. What is this and how is it helpful towards discovering more about your phage?
We would need to look at our restriction digest results again and re-compare them with already classified phages with similar results in order to make a new guess for its cluster and then run a PCR again. So short answer is we do not have a new prediction yet.
Hi!! It can’t necessarily prevent but it can lower the amount of resistant bacteria we have my lowering antibiotic usage by using phage therapy instead. Phage can co-evolve with bacteria which is why phage are able to attack and get rid of these resistant bacterias
curesymposium.org 
You found that the phage was not in the N or K4 cluster, do you have any other idea what cluster the phage may be in?
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You found that this phage was not in the N or K4 clusters, do you have any other idea of what cluster it might be in?
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Since you found that this phage is not in cluster N or K4, do you have any other idea(s) of what cluster it might be in?
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No, not yet. We would need to look at our restriction digest results again and compare those results again with already classified phages restriction digest results in order to make new predictions and then test those predictions on a PCR again.
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Hi! I thought your presentation was really good! I am wondering if there was a reason why you plan to run another PCR in the future instead of for this presentation. Going off of that, in what ways do you think you will modify your PCR if you were to do this again and how would that affect your results?
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We would run another PCR in order to hopefully find out what cluster our phage is in for sure. In the future we would modify our PCR by testing different clusters with our phages DNA in order to see if we get a positive result for one of those other clusters
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What were your negative and positive controls?
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Ou negative control is everything in the PCR mixture except for our predicted clusters (so our phage DNA and PCR reaction mix) and this should show nothing in our results. Our positive control is already classified phage DNA with its cluster (we used phage in the A4 cluster with A4 cluster primer mix) and this should show one band to show that it is correct.
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How would understanding what cluster your bacteriophage is in develop the research on a larger scale?
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It would help researchers find out what specific bacteria our phage is designed to attack and that would then help us know what bacterial infection our phage could be used for to treat in phage therapy.
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Hi! You mentioned something about ‘blast analysis’ at the end of your presentation. What is this and how is it helpful towards discovering more about your phage?
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Hi!
BLAST analysis is used to compare nucleotide or
protein sequences among different phages so its more geared to specific makeup of my phage.
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What cluster do you imagine the phage will be if not N or K? Have you had a chance to sequence the gene yet?
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We would need to look at our restriction digest results again and re-compare them with already classified phages with similar results in order to make a new guess for its cluster and then run a PCR again. So short answer is we do not have a new prediction yet.
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Have you had a chance to sequence the genome yet? (Sorry if this is a double comment the posting is weird)
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We have not had a chance to sequence our phage yet!
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Hi! How can phage therapy prevent multi-drug resistant bacterial infections?
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Hi!! It can’t necessarily prevent but it can lower the amount of resistant bacteria we have my lowering antibiotic usage by using phage therapy instead. Phage can co-evolve with bacteria which is why phage are able to attack and get rid of these resistant bacterias
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