Is there a specific type of PCR that you think would be more helpful going forward? Something like q-RT-PCR might help with the exploration and classification of your results in this lab.
Nice job. Are there any other possible tests you can run observing the characteristics of your chosen bacteriophage, just to further confirm which cluster the bacteriophage belongs to?
Nice job. My comment did not go through the first time. Are there any other tests you can run to determine any other characteristics your chosen bacteriophage possess (just to confirm further which clusters it actually belongs to)?
The plaques were measured using a ruler, and the phage itself was run through electron microscopy (figure 2), and the pictures had a scale (in nm) on them!
Were you expecting or hypothesizing that the phage would be a part of F1 or K4? Or was this simply just a surprise in the outcome of your research that was in your favor?
Initially, we did not hypothesize a cluster. After the restriction digest, we used the Phage Enzyme Tool to match up the amount of cuts on each enzyme, and then with consideration of plaque morphology we made predictions based on how the genomes appeared to match up. We then ran the PCR with samples of the predicted clusters, and it displayed the most similarity with F1 and K4.
If you’re familiar with phage therapy being used to combat otherwise untreatable antibiotic-resistant infections, I think that could be a big point of upcoming research. I also researched temperate phage being used to identify salmonella via bioluminescent genes, which appeared to hold promise in creating a diagnostic tool!
curesymposium.org 
Is there a specific type of PCR that you think would be more helpful going forward? Something like q-RT-PCR might help with the exploration and classification of your results in this lab.
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I’m unsure which type of PCR we would try next, however I would definitely like to repeat DNA isolation to ensure clear results first.
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Nice job. Are there any other possible tests you can run observing the characteristics of your chosen bacteriophage, just to further confirm which cluster the bacteriophage belongs to?
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Nice job. My comment did not go through the first time. Are there any other tests you can run to determine any other characteristics your chosen bacteriophage possess (just to confirm further which clusters it actually belongs to)?
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Thank you! The only way to know for sure which cluster would be to sequence the genome, which my lab partner is planning on doing next semester.
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What methods did you use to measure size of phage?
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The plaques were measured using a ruler, and the phage itself was run through electron microscopy (figure 2), and the pictures had a scale (in nm) on them!
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Were you expecting or hypothesizing that the phage would be a part of F1 or K4? Or was this simply just a surprise in the outcome of your research that was in your favor?
LikeLike
Initially, we did not hypothesize a cluster. After the restriction digest, we used the Phage Enzyme Tool to match up the amount of cuts on each enzyme, and then with consideration of plaque morphology we made predictions based on how the genomes appeared to match up. We then ran the PCR with samples of the predicted clusters, and it displayed the most similarity with F1 and K4.
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What do you think that the phage can be used for in future research?
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If you’re familiar with phage therapy being used to combat otherwise untreatable antibiotic-resistant infections, I think that could be a big point of upcoming research. I also researched temperate phage being used to identify salmonella via bioluminescent genes, which appeared to hold promise in creating a diagnostic tool!
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