One strong example of phage therapy being used and working is the articles referenced in the poster. In this example, phage therapy was used in a toddler in order to fight a bacterial infection of Pseudomonas aeruginosa. The phage therapy was successful enough that the toddler was able to safely undergo a liver transplant that saved their life.
The usage of a restriction digest and a PCR test would allow us to classify our phage into a specific cluster that would allow us to more accurately know what bacteria it is capable of infecting and destroying.
Why was your other enrichment unsuccessful and what led to the one you used being successful? What results would you expect or hope to get if you had had more time?
I believe our first enrichment was unsuccessful as the area the dirt sample was acquired from likely didn’t contain any of the proper phage needed. The second sample was taken from a similar location as many other successful enrichments in the class so it seems that we just got dirt from a better location the second go around. If we had more time we would like to have classified our phage into a specific cluster as well as having some of it’s DNA sequenced in order to better understand the phage.
Where you able to archive your phage and put it into the database?
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We were able to add our phage to the database as we met the base requirements. However, we weren’t able to add every image possible.
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What are some current examples of phages being used in treatment of bacteria infections or other areas?
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One strong example of phage therapy being used and working is the articles referenced in the poster. In this example, phage therapy was used in a toddler in order to fight a bacterial infection of Pseudomonas aeruginosa. The phage therapy was successful enough that the toddler was able to safely undergo a liver transplant that saved their life.
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How would a restriction digest and PCR tests further classify your phage?
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The usage of a restriction digest and a PCR test would allow us to classify our phage into a specific cluster that would allow us to more accurately know what bacteria it is capable of infecting and destroying.
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Why was your other enrichment unsuccessful and what led to the one you used being successful? What results would you expect or hope to get if you had had more time?
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I believe our first enrichment was unsuccessful as the area the dirt sample was acquired from likely didn’t contain any of the proper phage needed. The second sample was taken from a similar location as many other successful enrichments in the class so it seems that we just got dirt from a better location the second go around. If we had more time we would like to have classified our phage into a specific cluster as well as having some of it’s DNA sequenced in order to better understand the phage.
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