Since you said temperate phages aren’t used often for phage therapy, what would be your hopes for this compound if it were to continue going through future experimentation?
The hope is that temperate phages can be used in phage therapy more often due to their ability to be genetically modified to help lytic phages. I would hope to see more phage cocktails with temperate phages being used so more research can be conducted with temperate phages being used in phage therapy.
In your future directions you mention that you would like to isolate DNA from the phage and perform comparative analysis, why is this important to learning more about the temperate phage?
Thanks for your question! It is important to learn more about a phage like its DNA so we can see the enzymes that are in the phage. We can use the phages DNA for medical purposes and seeing if the phage is apart of a certain cluster based on the pcr test because certain clusters are common in certain bacteria viruses.
It is important to learn about the phages DNA so we can compare it to other clusters. Certain clusters are common with certain bacteria viruses, so learning more about the temperate phage can help us later with cocktail mixtures/solutions for phage therapy
You were talking about how purification needed to occur to prevent contamination of the phage. Do you know of any things specifically that could contaminate a phage?
Subatances that could be foreign to the phage like certain bacteria in the phage or even mistakes like dirt/spit/or even dust that got into the solution when working in the lab.
Good question, as for what I know about purification it allows us to get rid of any extra bacteria or contamination that may be in the soltuion so the phage that you already have is the phage that you are going to get after pruficaiton, but at the same time I do not fully know the anwser so I am not sure.
curesymposium.org 
Since you said temperate phages aren’t used often for phage therapy, what would be your hopes for this compound if it were to continue going through future experimentation?
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The hope is that temperate phages can be used in phage therapy more often due to their ability to be genetically modified to help lytic phages. I would hope to see more phage cocktails with temperate phages being used so more research can be conducted with temperate phages being used in phage therapy.
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In your future directions you mention that you would like to isolate DNA from the phage and perform comparative analysis, why is this important to learning more about the temperate phage?
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Thanks for your question! It is important to learn more about a phage like its DNA so we can see the enzymes that are in the phage. We can use the phages DNA for medical purposes and seeing if the phage is apart of a certain cluster based on the pcr test because certain clusters are common in certain bacteria viruses.
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It is important to learn about the phages DNA so we can compare it to other clusters. Certain clusters are common with certain bacteria viruses, so learning more about the temperate phage can help us later with cocktail mixtures/solutions for phage therapy
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sorry for the double reply, the first one did not load on my screen so i thought it didn’t comment
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You were talking about how purification needed to occur to prevent contamination of the phage. Do you know of any things specifically that could contaminate a phage?
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Subatances that could be foreign to the phage like certain bacteria in the phage or even mistakes like dirt/spit/or even dust that got into the solution when working in the lab.
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Is there a way to purify a temperate phage till you get the lysogenic phage you want?
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Good question, as for what I know about purification it allows us to get rid of any extra bacteria or contamination that may be in the soltuion so the phage that you already have is the phage that you are going to get after pruficaiton, but at the same time I do not fully know the anwser so I am not sure.
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