Interesting stuff. You mentioned that soybeans were a good candidate for the bio synthesis process for producing the needed proteins to make human collagen. How does doing the bio synthesis in soybeans compare to using another organism like yeast, as I have heard that is also a popular choice for large scale biosynthesis processes. Is one easier to make make or easier to scale up compared to the other?
Yeast could also be used to synthesize the collagen. However, the US already grows millions of acres of soybeans so the infrastructure for scaling biosynthesis is further along than in yeast.
You would take the amino acid sequence and run it through a computer program (like twist) to translate the amino acids into codons used most often by soybeans.
What is the difference between the collagen from soybeans and from animals? You mentioned that some people might have reactions to the collagen from animals because it isn’t human, could this happen from the collagen from the soybeans.
This would not happen with the collagen from the soybeans. The collagen encoded on the plasmid is human collagen, so the soybeans would produce human collagen. The human immune system can recognize proteins from other species, but since the collagen produced in soybeans is genetically human, the immune system won’t react.
Great presentation, I thought it was explained really well. Can you go into the golden gate assembly a little more? I am not sure of the mechanism fully.
You use restriction enzymes to cut the DNA. The insert will eventually bind to complementary ends in the new vector and a ligase will seal the backbone. Everything occurs in a single tube and you give the DNA time to assemble into the new vector where it cannot be cut out with restriction enzymes.
curesymposium.org 
Interesting stuff. You mentioned that soybeans were a good candidate for the bio synthesis process for producing the needed proteins to make human collagen. How does doing the bio synthesis in soybeans compare to using another organism like yeast, as I have heard that is also a popular choice for large scale biosynthesis processes. Is one easier to make make or easier to scale up compared to the other?
LikeLike
Yeast could also be used to synthesize the collagen. However, the US already grows millions of acres of soybeans so the infrastructure for scaling biosynthesis is further along than in yeast.
LikeLike
Loved your presentation! How exactly do you change the codons from human to soybean? Is it a reverse transcriptase process or something else?
LikeLike
You would take the amino acid sequence and run it through a computer program (like twist) to translate the amino acids into codons used most often by soybeans.
LikeLike
What is the difference between the collagen from soybeans and from animals? You mentioned that some people might have reactions to the collagen from animals because it isn’t human, could this happen from the collagen from the soybeans.
LikeLike
This would not happen with the collagen from the soybeans. The collagen encoded on the plasmid is human collagen, so the soybeans would produce human collagen. The human immune system can recognize proteins from other species, but since the collagen produced in soybeans is genetically human, the immune system won’t react.
LikeLike
Great presentation, I thought it was explained really well. Can you go into the golden gate assembly a little more? I am not sure of the mechanism fully.
LikeLike
You use restriction enzymes to cut the DNA. The insert will eventually bind to complementary ends in the new vector and a ligase will seal the backbone. Everything occurs in a single tube and you give the DNA time to assemble into the new vector where it cannot be cut out with restriction enzymes.
LikeLike