12 thoughts on “C16 – Olsen

    1. The PCR results for Hycop would appear with a brighter / greater intensity band in the hydroxyurea treated (+HU+RT) lane compared to the untreated (UT+RT) lane, as this would indicate a the expression of Hycop increased after DNA damage.

  1. Your conclusion is that Hycop does not play a role in DNA damage control but in the graph figure you have under ‘objectives’ they are super similar. do you think this conclusion would be different if the gels were loading correctly?

    1. Hi Julie, I hypothesize that the conclusion would still be the same if the gels were loaded correctly because the consensus gel, which is the general results obtained by other students researching this gene, also came to the same conclusion. However, it could be possible that the expression of Hycop decreased.

  2. Why do you think the bands were dimmer for Hycop compared to the controls? Is there a way you could alter your experiment that would make your results more conclusive?

    1. I think band for Hycop is dimmer because this gene is just not expressed as much in Tetrahymena thermophila as the loading control in general.

  3. Great presentation! What are the direct implications of understanding DNA repair mechanisms? Does it help us design better medications, treatments, etc?

    1. Any mutations or modifications that lead to faulty DNA repair mechanisms can result in diseases like cancer. Therefore, understanding the genes involved in this mechanism allows us to not only understand disease pathology, but also enable us to investigate and develop therapeutics.

  4. Great presentation! What are the direct benefits in treatment when we understand the DNA repair mechanisms? Does it help us develop better medications, etc?

    1. Hi Sarah, yes it can help us develop better medications and new approaches for treatments as understanding genes involved in the DNA repair mechanism strengthens how and why DNA repair defect diseases emerge.

    1. Hi Emery, we know the expression of Hycop is increased during conjugation state in Tetrahymena thermophila. Therefore, the only thing we can confirm is if Hycop is involved in DDR.

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