8 thoughts on “C22 – Connally

    1. During sexual reproduction, T. thermophila undergo segmentation of their genome. In order to maintain genome stability they need to possess the ability to repair their genome extremely well before finishing sexual reproduction. While I do not know the evolutionary influence to conduct genome segmentation during sexual reproduction, the evolution of DNA repair mechanisms was essential for the microbe to maintain genome stability.

    1. The specific mechanism of hydroxyurea was not discussed in class. However, hydroxyurea causes double-stranded DNA breaks, simulating the process of DNA damage during conjugation. This induces DNA repair mechanisms, allowing us to observe any difference in gene expression of Rfc1 with and without inducing DNA damage.

  1. Excellent presentation! You mention purifying the Rfc1 protein product in your future research. How will you use those isolated products? Will you try to identify other proteins expressed with Rfc1, or maybe introduce them into an Rfc1 knockout as a positive control?

    1. Rfc1 is a novel gene that has no previously determined function within T. thermophila. Homologs and a gene expression profile provided us with some insight as to whether the gene was a good candidate to study. The purpose for isolating Rfc1 protein product is to determine other protein products that associate with Rfc1. This could provide some information about Rfc1 gene expression relative to cell processes.

  2. Great Presentation! Just to clarify when you said “Slight expression increase in Rfc1 between untreated and hydroxyurea-treated.” you are saying that more rfc1 is expressed when the sample was treated with hydroxyurea? Would this mean that the hydroxyurea worked as a sort of stimulus for the rfc1?

  3. The predicted outcome of hydroxyurea treatment was that Rfc1 expression would increase relative to no treatment with hydroxyurea. This does mean that hydroxyurea acted as a sort of stimulus for Rfc1 which doesn’t guarantee that Rfc1 belongs to DNA repair pathways. That would have to be elucidated with future research concerning the function of Rfc1.

Leave a Reply