What do you think might have caused the untreated to be slightly brighter in the RppO primer results? I know you mentioned that it can happen sometimes, Does it have any major effect to the overall project?
For this experiment the Rpp0 primer acted as our loading control to make sure the same amount of cDNA was added to our treated and untreated samples. This may have indicated that slightly more cDNA was added to the untreated sample. This could have also been caused by something else so I am not exactly sure why this occurred. However for our quantitative analysis the measurements were normalized to the loading control samples so this got rid of that variable in our results.
We did not actually measure the rate at which the DNA damage was repaired. However our project was to look at sRNA associated protein Dicer-2 and see if this protein enables the sRNAs it is associated with to repair damaged DNA. When DNA damage was induced we measured if this gene increases in expression indicating that it might have some involvement in DNA damage repair.
While researching Dicer-2 we found that its closest homologs were in soy beans. It did have some homologs in humans but they weren’t very close. I am not actually sure if this repair pathway research could be carried over into humans. However I do think it could be researched further to fully understand the repair pathway in T. thermophila and since its DNA is highly conserved it might show insight into some other related organisms.
In the future directions, you mentioned that you could potentially do more experiments to better understand what exactly dcr2 does. What kinds of experiments would that entail?
curesymposium.org 
What do you think might have caused the untreated to be slightly brighter in the RppO primer results? I know you mentioned that it can happen sometimes, Does it have any major effect to the overall project?
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For this experiment the Rpp0 primer acted as our loading control to make sure the same amount of cDNA was added to our treated and untreated samples. This may have indicated that slightly more cDNA was added to the untreated sample. This could have also been caused by something else so I am not exactly sure why this occurred. However for our quantitative analysis the measurements were normalized to the loading control samples so this got rid of that variable in our results.
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How do you measure DNA damage repair rate?
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We did not actually measure the rate at which the DNA damage was repaired. However our project was to look at sRNA associated protein Dicer-2 and see if this protein enables the sRNAs it is associated with to repair damaged DNA. When DNA damage was induced we measured if this gene increases in expression indicating that it might have some involvement in DNA damage repair.
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What potential practical uses could this research serve, now that we know there is a connection?
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While researching Dicer-2 we found that its closest homologs were in soy beans. It did have some homologs in humans but they weren’t very close. I am not actually sure if this repair pathway research could be carried over into humans. However I do think it could be researched further to fully understand the repair pathway in T. thermophila and since its DNA is highly conserved it might show insight into some other related organisms.
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How did you induce damage to the DNA?
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We induced DNA damage by adding the chemical hydroxyurea to T. thermophila cells. This chemical causes a lot of double stranded breaks in the DNA.
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In the future directions, you mentioned that you could potentially do more experiments to better understand what exactly dcr2 does. What kinds of experiments would that entail?
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What may be the reason why the untreated is more white?
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