8 thoughts on “C31 – Ogren

  1. In one of your trials (figure four) what does it mean when something appears dimmer, I was a little lost when you were discussing your results from that trial? Did your trials that you put on the poster/discussed connected to eachother or different trials (figure four and both figure 5a and 5b)? Great poster

    1. Usually when a band is dimmer, it means there is less expression of whatever is being measured. I think what you are pointing out in figure 4 is that the gDNA band is brighter than the cDNA band. This could simply be because there is more gDNA than cDNA in our PCR samples, but more experiments would need to be done to confirm or deny this. Figures 5a and 5b are connected to each other. 5b was the quantitative analysis taken from measuring the brightness in the bands from figure 5a to help us better visualize the results.

  2. Are there other organisms that perform similar amounts of conjugation, if so, why was T. thermophilia chosen?

    1. T. Thermophilia was chosen because it causes DNA damage naturally during conjugation. Because it causes a lot of DNA damage to it’s cells and our goal was to study what cells are involved in the DNA damage repair pathway, it served as a perfect model organism. I do not know of any other specific organisms that causes DNA damage during conjugation, but T.thermopila is also widely distributed in labs making it easy to access.

  3. What is a gene similar to T. thermophilia that could be a possible alternative for DNA damage repair?

    1. T. thermophilia was our model organism. The gene we studied was Upec which is found in T. Thermophilia and is expressed during conjugation. There are currently no domains for our gene, meaning that there are no other genes in other organisms completely like it. There are many other genes (many of which were studied by my classmates) that are also expressed during T. Thermopilia conjugation, and that are involved in the DNA damage repair pathway. These genes participate in DNA damage repair whereas Upec does not. To find genes that are an alternative to Upec there would need to be more experiments to either find domains for Upec or modify the gene itself to aid in DNA damage repair.

  4. How could you test your new hypothesis about Upec being an antagonist to typical repair mechanisms?

    1. Great question! In order to test this we could run an experiment where we knockout Upec from the genome entirely and then compare the amount of DNA damage repair that occurs with Upec and without. An experiment like this might give more background as to what the exact function of Upec might be. If without Upec there are higher amounts of DNA damage repair, this may show that Upec actually does something to inhibit or regulate DNA damage repair and is an antagonist to typical repair mechanisms. If there is a different result it may suggest that Upec plays a different role when it is expressed during conjugation.

Leave a Reply