8 thoughts on “C43 – Espey, Z.

    1. Hi Ariana, thank you for your question. To start, Hypzif is a gene unique to Tetrahymena Thermophila, which is a single-celled eukaryote, not a bacterium. Data analysis has shown that the Hypzif gene is not found in any other organisms other than Tetrahymena, and therefore it would not be possible to study it in other single-celled organisms similar to T. thermophila.

  1. Could the peaks seen in the microarray possibly suggest that Hypzif is involved in the creation of the DSBs as opposed to their repair?

    1. Hi Elle! Thank you for the question. It is possible that that may be the case as conjugation is the period in which those double stranded breaks occur. I’m not sure what the exact mechanism is that Tetrahymena uses to induce double stranded breaks, but that hypothesis is viable too.

    1. Hi Abby! Great question. The indication for DNA damage in our experiment is a change in expression of the gene Rad51. Rad51 is a gene known to play a role in DNA repair in Tetrahymena Thermophila, and therefore if we see a higher amount of expression in cells treated with a DNA damaging agent compared to cells that are untreated, we can confidently say that DNA damage has occurred.

  2. Thanks for your work! Was there any reason why you chose this gene in particular to study rather than others that might be involved in the DNA damage response pathway?

    1. Hi Christopher! Hypzif was selected because its gene expression profile is similar to the expression profile of genes known to be involved in the DNA damage response pathway, like Rad51. Compared to other genes possibly involved in DNA repair, there is no great difference between them! They all show these expression profiles, and therefore each needs to be tested.

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