9 thoughts on “C49 – Juarez

  1. How does hydroxyurea induce DNA damage? Is it dangerous for researchers to come in contact with?

    1. Hydroxyurea induce DNA damage by the formation of nitric oxide and hydrogen peroxide. Yes, it can be dangerous for researchers to come in contact with, it could lower white blood cell count.

    2. It induces DNA damage by forming nitric oxide and hydrogen peroxide. It can be dangerous for researchers to come in contact with due to the possibility of it lowering your white blood cell count when coming in contact.

    1. An possible error that could arise is the our DNA ladder being over run during gel electrophoresis which would leave our ladder bands smudges. This would make it hard for our find the length of the genes or if the genes annealed to the correct place. Another possible error is contamination which would affect the results by not knowing if all the samples are contaminated or just that one well.

  2. Why did you choose to use the Sams primer after the first round of primers you used showed no results? What properties did it have that made it an optimistic option for different results?

    1. We choose to go with Sams primer because our designed primer, showed signs of poor annealing with the low expression and the incorrect placement of annealing on the ladder. We were optimistic about using Sams because it was already a verified primer which means it would work better then our primers who showed signs of low annealing.

  3. Are there any dangers or health concerns while doing this research for cancer and repairing damaged genes and DNA?

    1. The only danger that comes to mind, is the use of hydroxyurea when confirming if the t. theromphilla gene is involved in DNA damage repair.

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