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Could a potential application involve cancer prevention since an up-regulation of DNA repair pathways could minimize the potential of DNA mutations that confer cancer? LikeLike Reply
Because the cDNA sequence is not highly expressed in cells not undergoing DNA damage. The primers were tested with untreated DNA LikeLike Reply
Why do you think the band for your gene was fainter than the band for RAD51 when DNA damage was induced? LikeLike Reply
I believe this is because Rad51 is more highly expressed in the repair pathway than Hyplrr. Hyplrr way not be playing as big of a role as Rad51 is LikeLike Reply
The hope is to be able to use these highly accurate repair proteins in treatment of diseases associated with fault DNA damage repair LikeLike Reply
Could a potential application involve cancer prevention since an up-regulation of DNA repair pathways could minimize the potential of DNA mutations that confer cancer?
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That is the hope!
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Why do you think your primers were unable to anneal to the cDNA sequence?
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Because the cDNA sequence is not highly expressed in cells not undergoing DNA damage. The primers were tested with untreated DNA
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Why do you think the band for your gene was fainter than the band for RAD51 when DNA damage was induced?
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I believe this is because Rad51 is more highly expressed in the repair pathway than Hyplrr. Hyplrr way not be playing as big of a role as Rad51 is
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What is the “big picture” of your finding in the future?
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The hope is to be able to use these highly accurate repair proteins in treatment of diseases associated with fault DNA damage repair
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