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14 thoughts on “C50 – Parisi”
Great presentation! How did you degrade the DNA to test if that gene was involved in DNA repair?
We induced DNA damage and measured if there was an increase of expression. An increase of expression could indicate that Socax is involved in DNA repair pathways. I am pretty sure we chemically induced DNA damage.
What is your main conclusion about Socax after induced DNA damage?
Since there were no bands present during the second round of PCR and gel electrophoresis I could not conclude whether Socax is involved in the DDR pathway or not. Further testing should be done.
Very interesting presentation! How did you design the primers needed to conduct the experiment?
I designed forward and reverse primers in Primer3plus!
Why did you choose Socax as your testing model? Are there any examples of prior research on Socax produced by other scientists?
I was assigned the hypothetical gene Socax. Socax had similar gene expression patterns to known genes that are involved in the DNA repair pathway, which is why it was a good candidate for study. I am unaware if there is any prior experiments done on Socax.
Hi, great job presenting ! I am kind of confused, if your experiment had worked, how would you have compared the positive and negative controls to your tested sample ?
The positive control in the experiment was inducing DNA damage to Rad51. Rad51 is known to be involved in DNA repair pathways and show an increase in gene expression when DNA damage was induced. The negative controls were the last three lanes. I used Rad51, Ftt18 and my gene Socax with no reverse transcriptase to rule out genomic DNA contamination. There should be no bands present in these lanes. If my gene Socax had bands present when DNA damage was induced, Rad51 had an increase in gene expression and my -RT lanes had no bands present, I would have been able to confirm that the bands present in the Socax lanes were not from contamination or poor experimental procedures.
Is there any models that are similar to Socax that you could use for futher research? What has been tested previously on this model? What were those outcomes?
There were no homologs for the Socax gene, so I am unaware if there any other genes that are very similar to Socax. I am not sure what has been tested previously on this model or what the outcomes could have been.
If you could redo this experiment what would you use instead of socax and why?
Since the gene Socax was assigned to me, I would like to redo this experiment with a gene that has a human homolog to hopefully use that data for cancer research.