8 thoughts on “C57 – Brown

  1. Can you further explain why you chose the gene you examined? I understand you chose it because of mRNA expression levels closely relating to genes that exist in DNA, but didn’t hear what you said after DNA… (Time was 1:59)

    1. Apologies for my mic quality! I basically said that the mRNA expression levels increase during conjugation (DNA damage occurs during this process), and known DNA damage repair genes such as rad51 follow this pattern. Since both of these gene share similar expression patterns, it makes setk a candidate for DNA damage repair.

    1. An immunofluorescent experiment could allow us to visualize exactly where in the cell the setk product is being expressed, which can provide more context on it’s function. This would involve injecting antibodies against the setk protein with an attached fluorophore. These antibodies would associate with the setk product and fluoresce

  2. I understand the hypothesis that this gene might play a role in DNA damage repair, however the resulted concluded this is unlikely. What are some other possible function of this gene if its located in the phase were DNA damage repair occurs?

    1. Based on the gene sequence similarity to other proteins (using BLAST), setk is similar to a kinase protein (serine threonine kinase related protein). This would indicate that the protein product of setk likely is involved in the transfer of phosphate groups. The peaks of mRNA expression occur during the conjugation phase of Tetrahymena. While DNA damage does occur here, the cell is also working to reproduce. So setk likely plays some role during that process.

  3. Great presentation & great explanation of results / future directions. If this protein activated a signaling pathway that activated / recruited other proteins directly involved in DNA damage response, would greater levels of expression of this protein need to be present to confirm it is directly involved in DNA damage response?

    1. Thank you! Yes, we would expect some level of increased expression, even if it’s a regulatory gene. The only 2 situation that I could think of that would not increase would be if this gene encoded for a repressor, or if it’s not involved in the pathway.

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