6 thoughts on “C6 – Capes

    1. Thanks for watching! So the BART domain is known to bind to ARL2 and ARL3. While this domain is not entirely well studied this aspect of it is well known. So while we know that Badcp isn’t involved in DNA repair, we would still like to find the function of the gene. With the presence of the BART domain, we would hope to be able to use this as a starting point to identify what exactly the Badcp gene is involved in inside the cell and further the study of what the function of both the BART domain and the Badcp gene are.

    1. Thanks for watching, and the kind words! So when we induced damage to the cell, we used the hydroxyurea to actually damage the DNA inside the cell. It did this by creating double strand breaks to the DNA inside the cell.

  1. Is there a way we could use this gene to create a therapy for genes that do get damaged easily? Also, is this gene highly important in the body and is it good that it does not get damaged? Slightly confused on what the hypothesis for testing this gene was! Overall it was really good though, nice and clear!

    1. Thanks for watching! At the moment it is looking like no, we cannot use this as a therapy for genes that are damaged easily. All DNA is at similar risk for damage when it is induced, however, some genes are involved in the DNA repair pathway and will act to fix damage in the cell when it occurs. The main purpose of this experiment was to discover whether or not Badcp is one of these genes. So Badcp and other genes don’t ‘resist’ damage, but rather it will be repaired when it occurs. And to clarify your last question, since we saw that Badcp was up-regulated during replication (when damage occurs in T. thermophila). we believed that Badcp might be involved in DNA damage repair. So our hypothesis was that “if Badcp is involved in DNA damage repair, then Badcp expression will increase after DNA damage is induced to the cell”. Sorry for replying with so much, but I hope that helps!

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